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Literature summary extracted from

  • Hovingh, P.; Linker, A.
    The enzymatic degradation of heparin and heparitin sulfate. 3. Purification of a heparitinase and a heparinase from flavobacteria (1970), J. Biol. Chem., 245, 6170-6175.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.7 Cu2+
-
 Flavobacteriia
4.2.2.7 Fe3+
-
 Flavobacteriia
4.2.2.7 Hg2+
-
 Flavobacteriia
4.2.2.8 Cd2+ 1 mM Flavobacteriia
4.2.2.8 Cu2+ 1 mM Flavobacteriia
4.2.2.8 Hg2+ 1 mM Flavobacteriia
4.2.2.8 N-acetylheparin
-
 Flavobacteriia
4.2.2.8 Zn2+ 1 mM Flavobacteriia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.7 Ca2+ slight stimulation Flavobacteriia
4.2.2.7 Mn2+ slight stimulation Flavobacteriia
4.2.2.8 Cd2+ 0.01 mM, stimulates Flavobacteriia
4.2.2.8 Cu2+ 0.01 mM, stimulates Flavobacteriia
4.2.2.8 Hg2+ 0.01 mM, stimulates Flavobacteriia

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.7 Flavobacteriia
-
-
-
4.2.2.8 Flavobacteriia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.7
-
 Flavobacteriia
4.2.2.8
-
 Flavobacteriia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.7 additional information
-
-
 Flavobacteriia

Storage Stability

EC Number Storage Stability Organism
4.2.2.7 1 month at -15°C Flavobacteriia
4.2.2.7 At 4°C, some loss of activity after 5 weeks Flavobacteriia
4.2.2.8 4°C, for at least 2 months Flavobacteriia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.7 heparin
-
 Flavobacteriia unsaturated heparin disaccharide + unsaturated heparin tetrasaccharide + unsaturated heparin hexasaccharide
-
 ?
4.2.2.7 Heparin sulfate heparin-like portion Flavobacteriia ?
-
 ?
4.2.2.7 additional information requirement O-sulfate and sulfamido groups Flavobacteriia additional information
-
 ?
4.2.2.8 heparitin sulfates and related compounds Flavobacteriia ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.7 31
-
-
 Flavobacteriia
4.2.2.8 42
-
-
 Flavobacteriia

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.7 25 40
-
 Flavobacteriia
4.2.2.8 22 52
-
 Flavobacteriia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.7 6.5
-
-
 Flavobacteriia
4.2.2.8 6.8
-
-
 Flavobacteriia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.7 5 9
-
 Flavobacteriia