EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.98 | SDS | 0.1%, stimulates | Eubacterium sp. | |
4.2.1.98 | SDS | 0.05%, about 7fold stimulation | Eubacterium sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.98 | 16-Ketoprogesterone | competitive with respect to 16alpha-hydroxypregnenolone and 16alpha-hydroxyprogesterone | Eubacterium sp. | |
4.2.1.98 | 16alpha-hydroxypregnenolone | substrate inhibition at high concentrations | Eubacterium sp. | |
4.2.1.98 | cetyltrimethylammonium bromide | no inhibition by NaBr | Eubacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.98 | 0.25 | - |
16alpha-hydroxypregnenolone | - |
Eubacterium sp. | |
4.2.1.98 | 0.52 | - |
16alpha-hydroxyprogesterone | - |
Eubacterium sp. | |
4.2.1.98 | 0.52 | - |
16alpha-hydroxyprogesterone | pH 6.8 | Eubacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 42400 | - |
4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
4.2.1.98 | 42400 | - |
8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
4.2.1.98 | 42400 | - |
x * 42400, SDS-PAGE | Eubacterium sp. |
4.2.1.98 | 181000 | - |
tetrameric form, gel filtration | Eubacterium sp. |
4.2.1.98 | 181000 | - |
active in two molecular weight forms, gel filtration | Eubacterium sp. |
4.2.1.98 | 326000 | - |
octameric form, gel filtration | Eubacterium sp. |
4.2.1.98 | 326000 | - |
active in two molecular weight forms, gel filtration | Eubacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.98 | Eubacterium sp. | - |
- |
- |
4.2.1.98 | Eubacterium sp. 144 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.98 | - |
Eubacterium sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 0.7 | - |
- |
Eubacterium sp. |
4.2.1.98 | 0.7094 | - |
- |
Eubacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.98 | 16alpha-Hydroxypregnenolone | - |
Eubacterium sp. | ? | - |
? | |
4.2.1.98 | 16alpha-Hydroxypregnenolone | - |
Eubacterium sp. 144 | ? | - |
? | |
4.2.1.98 | 16alpha-hydroxypregnenolone | - |
Eubacterium sp. | 16-dehydropregnenolone + H2O | - |
? | |
4.2.1.98 | 16alpha-hydroxypregnenolone | - |
Eubacterium sp. 144 | 16-dehydropregnenolone + H2O | - |
? | |
4.2.1.98 | 16alpha-Hydroxyprogesterone | - |
Eubacterium sp. | 16-Dehydroprogesterone | - |
? | |
4.2.1.98 | 16alpha-Hydroxyprogesterone | - |
Eubacterium sp. 144 | 16-Dehydroprogesterone | - |
? | |
4.2.1.98 | 16alpha-hydroxyprogesterone | - |
Eubacterium sp. | 16dehydroprogesterone + H2O | - |
? | |
4.2.1.98 | 16alpha-hydroxyprogesterone | - |
Eubacterium sp. 144 | 16dehydroprogesterone + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.98 | ? | x * 42400, SDS-PAGE | Eubacterium sp. |
4.2.1.98 | octamer | 8 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
4.2.1.98 | tetramer | 4 * 42400, the enzyme is functional as tetramer and as octamer, SDS-PAGE | Eubacterium sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.98 | 0.08 | - |
16-Ketoprogesterone | pH 6.8, reaction with 16alpha-hydroxyprogesterone | Eubacterium sp. | |
4.2.1.98 | 0.18 | - |
16-Ketoprogesterone | pH 6.8, reaction with 16alpha-hydroxypregnenolone | Eubacterium sp. |