EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.96 | D100N | the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme | Pseudomonas aeruginosa |
4.2.1.96 | E69A | the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme | Pseudomonas aeruginosa |
4.2.1.96 | H73A | the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme | Pseudomonas aeruginosa |
4.2.1.96 | H73A/H74A | the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme | Pseudomonas aeruginosa |
4.2.1.96 | H74A | the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme | Pseudomonas aeruginosa |
4.2.1.96 | H91A | the single mutants H73A and H74A and the double mutant H73A,H74A are completely inactive. The activity of the mutant enzymes H91A and E69A is 40% of the activity of the wild type enzyme. The activity of the mutant enzymes D100N is 10% of the activity of the wild type enzyme | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.96 | 0.008 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme Y69F | Homo sapiens | |
4.2.1.96 | 0.01 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme H79A | Homo sapiens | |
4.2.1.96 | 0.018 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme D60A | Homo sapiens | |
4.2.1.96 | 0.02 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | - |
Homo sapiens | |
4.2.1.96 | 0.02 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | wild type enzyme and mutant enzyme G72D | Pseudomonas aeruginosa | |
4.2.1.96 | 0.025 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | wild type enzyme | Homo sapiens | |
4.2.1.96 | 0.025 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme E69A | Pseudomonas aeruginosa | |
4.2.1.96 | 0.06 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme H91A | Pseudomonas aeruginosa | |
4.2.1.96 | 0.075 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme H62A | Homo sapiens | |
4.2.1.96 | 2.2 | - |
6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | mutant enzyme H61A | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.96 | Homo sapiens | - |
wild type and mutant enzymes E57A, H61A, H62A, H79A, Y69F, and D60A and double-mutant H61A,H62A expressed in Escherichia coli | - |
4.2.1.96 | Pseudomonas aeruginosa | - |
wild type and mutant enzymes E69A, D100N, H73A, H74A, H73A,H74A, H91A, W81A, and G72S expressed in Escherichia coli | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.96 | wild type and mutant enzymes E69A, D100N, H73A, H74A, H91A, W81A, and G72S and double-mutant H73A,H74A expressed in Escherichia coli | Pseudomonas aeruginosa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.1.96 | 4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H2O | mechanism | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.96 | 4a-hydroxy-tetrahydropterin | - |
Homo sapiens | 7,8-dihydropterin + H2O | - |
? | |
4.2.1.96 | 4a-hydroxy-tetrahydropterin | - |
Pseudomonas aeruginosa | 7,8-dihydropterin + H2O | - |
? | |
4.2.1.96 | 6(S)-methyl-4a-hydroxy-tetrahydropterin | - |
Pseudomonas aeruginosa | 6(S)-methyl-7,8-dihydropterin + H2O | - |
? | |
4.2.1.96 | 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | - |
Homo sapiens | ? | - |
? | |
4.2.1.96 | 6(S)-methyl-7,8-dihydropterin-4a-carbinolamine | - |
Pseudomonas aeruginosa | ? | - |
? |