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Literature summary extracted from

  • Hösel, W.; Schiel, O.
    Biosynthesis of cyanogenic glucosides: in vitro analysis of the glucosylation step (1984), Arch. Biochem. Biophys., 229, 177-186.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.178 0.5
-
 UDP-glucose 4-hydroxymandelonitrile or 3,4-dihydroxymandelonitrile Triglochin maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.178 soluble
-
 Triglochin maritima
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.178 High salt concentrations 160% activity observed at 0.6 M concentration of NaCl, KCl or ammonium sulfate with 4-hydroxymandelonitrile, and 20% activity observed at 0.1-0.2 M concentration with 3,4-dihydroxymandelonitrile Triglochin maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.178 47000
-
 1 * 47000, SDS-PAGE Triglochin maritima
2.4.1.178 50000
-
 gel filtration Triglochin maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.178 UDP-glucose + 4-hydroxymandelonitrile Triglochin maritima last step in biosynthesis of cyanogenic glucosides UDP + taxiphyllin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.178 Triglochin maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.178 method that includes chromatography on Sephadex PD10 and DEAE-Sephadex Triglochin maritima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.178 seedling
-
 Triglochin maritima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.178 0.31
-
 3,4-dihydroxymandelonitrile Triglochin maritima
2.4.1.178 0.94
-
 4-hydroxymandelonitrile Triglochin maritima

Storage Stability

EC Number Storage Stability Organism
2.4.1.178 -20°C, 10% glycerol, crude extract stable for several weeks Triglochin maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.178 UDP-glucose + 3,4-dihydroxymandelonitrile
-
 Triglochin maritima UDP + 3,4-dihydroxymandelonitrile glucoside
-
 ?
2.4.1.178 UDP-glucose + 4-hydroxymandelonitrile
-
 Triglochin maritima UDP + taxiphyllin
-
 ?
2.4.1.178 UDP-glucose + 4-hydroxymandelonitrile last step in biosynthesis of cyanogenic glucosides Triglochin maritima UDP + taxiphyllin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.178 monomer 1 * 47000, SDS-PAGE Triglochin maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.178 30
-
-
 Triglochin maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.178 6.5 8.5
-
 Triglochin maritima
2.4.1.178 8
-
 assay at Triglochin maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.178 6 9.5 about 50% of activity maximum at pH 6 and pH 9.5 Triglochin maritima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.178 7.5
-
 unstable below, crude enzyme extract Triglochin maritima