Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Amano, J.; Kobata, A.
    Purification and characterization of a novel alpha-mannosidase from Aspergillus saitoi (1986), J. Biochem., 99, 1645-1654.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 Cu2+ 1 mM, 50% inhibition Aspergillus phoenicis
3.2.1.114 additional information not inhibited by EDTA Aspergillus phoenicis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.24 1.13
-
 Man3GlcNAc2
-
 Aspergillus phoenicis
3.2.1.114 1.25
-
 Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc NaBH4 reduced Aspergillus phoenicis
3.2.1.114 1.25
-
 Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc pH 5, 37°C Aspergillus phoenicis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Ca2+ strong activation Aspergillus phoenicis
3.2.1.114 Ca2+ strongly activates, 1 mM, 13fold Aspergillus phoenicis
3.2.1.114 additional information not activated by Zn2+ Aspergillus phoenicis
3.2.1.114 additional information not activated by Ba2+ Aspergillus phoenicis
3.2.1.114 additional information not activated by Mg2+, Mn2+ Aspergillus phoenicis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 Man3GlcNAc2 + H2O Aspergillus phoenicis Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed Man2GlcNAc2 + alpha-D-mannopyranose
-
 ?
3.2.1.24 Man5GlcNAc2 + H2O Aspergillus phoenicis cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages Man4GlcNAc2 + alpha-D-mannopyranose mixture of three isomeric oligosaccharides ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Aspergillus phoenicis
-
-
-
3.2.1.114 Aspergillus phoenicis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Aspergillus phoenicis
3.2.1.114 1000fold Aspergillus phoenicis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 cell culture
-
 Aspergillus phoenicis
-
3.2.1.114 mycelium freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin Aspergillus phoenicis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.114 38000
-
-
 Aspergillus phoenicis

Storage Stability

EC Number Storage Stability Organism
3.2.1.24 4°C or -20°C, 4 months Aspergillus phoenicis
3.2.1.114 -20°C or 4°C, 4 months, less than 5% loss of activity Aspergillus phoenicis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 Man3GlcNAc2 + H2O Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed Aspergillus phoenicis Man2GlcNAc2 + alpha-D-mannopyranose
-
 ?
3.2.1.24 Man5GlcNAc2 + H2O cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages Aspergillus phoenicis Man4GlcNAc2 + alpha-D-mannopyranose mixture of three isomeric oligosaccharides ?
3.2.1.114 D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O NaBH4 reduced, poor substrate Aspergillus phoenicis ?
-
 ?
3.2.1.114 D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O NaBH4 reduced, good substrate Aspergillus phoenicis D-Manbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced ?
3.2.1.114 D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis Aspergillus phoenicis D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced ?
3.2.1.114 GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too Aspergillus phoenicis ?
-
 ?
3.2.1.114 GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis Aspergillus phoenicis ?
-
 ?
3.2.1.114 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced Aspergillus phoenicis Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced ?
3.2.1.114 Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O NaBH4 reduced, cleaves the Manalpha(1-6)Man linkage only after its Manalpha(1-3) residue is removed Aspergillus phoenicis Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose NaBH4 reduced, no product: Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc ?
3.2.1.114 additional information not: 4-nitrophenyl alpha-mannoside Aspergillus phoenicis ?
-
 ?
3.2.1.114 additional information not: D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-4))[GlcNAcbeta(1-4)(GlcNAcbeta(1-2))Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-4)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, all sodium borate reduced Aspergillus phoenicis ?
-
 ?
3.2.1.114 additional information substrate specificity, hydrolysis mechanism of branched oligosaccharides Aspergillus phoenicis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.114 37
-
 assay at Aspergillus phoenicis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 5
-
-
 Aspergillus phoenicis
3.2.1.114 5
-
-
 Aspergillus phoenicis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.114 3.5 6 pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity Aspergillus phoenicis