BRENDA - Enzyme Database

Purification and characterization of a novel alpha-mannosidase from Aspergillus saitoi

Amano, J.; Kobata, A.; J. Biochem. 99, 1645-1654 (1986) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.114
Cu2+
1 mM, 50% inhibition
Aspergillus phoenicis
3.2.1.114
additional information
not inhibited by EDTA
Aspergillus phoenicis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.24
1.13
-
Man3GlcNAc2
-
Aspergillus phoenicis
3.2.1.114
1.25
-
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc
NaBH4 reduced
Aspergillus phoenicis
3.2.1.114
1.25
-
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc
pH 5, 37°C
Aspergillus phoenicis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.24
Ca2+
strong activation
Aspergillus phoenicis
3.2.1.114
Ca2+
strongly activates, 1 mM, 13fold
Aspergillus phoenicis
3.2.1.114
additional information
not activated by Zn2+
Aspergillus phoenicis
3.2.1.114
additional information
not activated by Ba2+
Aspergillus phoenicis
3.2.1.114
additional information
not activated by Mg2+, Mn2+
Aspergillus phoenicis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.24
Man3GlcNAc2 + H2O
Aspergillus phoenicis
Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
Man2GlcNAc2 + alpha-D-mannopyranose
-
Aspergillus phoenicis
?
3.2.1.24
Man5GlcNAc2 + H2O
Aspergillus phoenicis
cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
Man4GlcNAc2 + alpha-D-mannopyranose
mixture of three isomeric oligosaccharides
Aspergillus phoenicis
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.24
Aspergillus phoenicis
-
-
-
3.2.1.114
Aspergillus phoenicis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.24
to homogeneity, chromatography steps
Aspergillus phoenicis
3.2.1.114
1000fold
Aspergillus phoenicis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.24
cell culture
-
Aspergillus phoenicis
-
3.2.1.114
mycelium
freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin
Aspergillus phoenicis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.114
38000
-
-
Aspergillus phoenicis
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.24
4°C or -20°C, 4 months
Aspergillus phoenicis
3.2.1.114
-20°C or 4°C, 4 months, less than 5% loss of activity
Aspergillus phoenicis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.24
Man3GlcNAc2 + H2O
Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
586942
Aspergillus phoenicis
Man2GlcNAc2 + alpha-D-mannopyranose
-
586942
Aspergillus phoenicis
?
3.2.1.24
Man5GlcNAc2 + H2O
cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
586942
Aspergillus phoenicis
Man4GlcNAc2 + alpha-D-mannopyranose
mixture of three isomeric oligosaccharides
586942
Aspergillus phoenicis
?
3.2.1.114
D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, poor substrate
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, good substrate
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
3.2.1.114
D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
3.2.1.114
GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced
586942
Aspergillus phoenicis
Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
3.2.1.114
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, cleaves the Manalpha(1-6)Man linkage only after its Manalpha(1-3) residue is removed
586942
Aspergillus phoenicis
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced, no product: Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
586942
Aspergillus phoenicis
?
3.2.1.114
additional information
not: 4-nitrophenyl alpha-mannoside
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
additional information
not: D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-4))[GlcNAcbeta(1-4)(GlcNAcbeta(1-2))Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-4)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, all sodium borate reduced
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
additional information
substrate specificity, hydrolysis mechanism of branched oligosaccharides
586942
Aspergillus phoenicis
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.114
37
-
assay at
Aspergillus phoenicis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.24
5
-
-
Aspergillus phoenicis
3.2.1.114
5
-
-
Aspergillus phoenicis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.114
3.5
6
pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity
Aspergillus phoenicis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.114
Cu2+
1 mM, 50% inhibition
Aspergillus phoenicis
3.2.1.114
additional information
not inhibited by EDTA
Aspergillus phoenicis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.24
1.13
-
Man3GlcNAc2
-
Aspergillus phoenicis
3.2.1.114
1.25
-
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc
NaBH4 reduced
Aspergillus phoenicis
3.2.1.114
1.25
-
Manalpha(1,6)(Manalpha(1,3))Manbeta(1,4)GlcNAcbeta(1,4)GlcNAc
pH 5, 37°C
Aspergillus phoenicis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.24
Ca2+
strong activation
Aspergillus phoenicis
3.2.1.114
Ca2+
strongly activates, 1 mM, 13fold
Aspergillus phoenicis
3.2.1.114
additional information
not activated by Zn2+
Aspergillus phoenicis
3.2.1.114
additional information
not activated by Ba2+
Aspergillus phoenicis
3.2.1.114
additional information
not activated by Mg2+, Mn2+
Aspergillus phoenicis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.24
Man3GlcNAc2 + H2O
Aspergillus phoenicis
Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
Man2GlcNAc2 + alpha-D-mannopyranose
-
Aspergillus phoenicis
?
3.2.1.24
Man5GlcNAc2 + H2O
Aspergillus phoenicis
cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
Man4GlcNAc2 + alpha-D-mannopyranose
mixture of three isomeric oligosaccharides
Aspergillus phoenicis
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.24
to homogeneity, chromatography steps
Aspergillus phoenicis
3.2.1.114
1000fold
Aspergillus phoenicis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.24
cell culture
-
Aspergillus phoenicis
-
3.2.1.114
mycelium
freeze-dried mixture of mycelium and its culture medium, commercially available as Morushin
Aspergillus phoenicis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.114
38000
-
-
Aspergillus phoenicis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.24
4°C or -20°C, 4 months
Aspergillus phoenicis
3.2.1.114
-20°C or 4°C, 4 months, less than 5% loss of activity
Aspergillus phoenicis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.24
Man3GlcNAc2 + H2O
Man-1,6-linkage only hydrolysed after its Man-1,3-residue is removed
586942
Aspergillus phoenicis
Man2GlcNAc2 + alpha-D-mannopyranose
-
586942
Aspergillus phoenicis
?
3.2.1.24
Man5GlcNAc2 + H2O
cleaves Man-alpha-(1-3)-linkage 10times faster than the Man-1,6 and the Man-1,2-linkages
586942
Aspergillus phoenicis
Man4GlcNAc2 + alpha-D-mannopyranose
mixture of three isomeric oligosaccharides
586942
Aspergillus phoenicis
?
3.2.1.114
D-Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, poor substrate
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
D-Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
NaBH4 reduced, good substrate
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
3.2.1.114
D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, at lower rate than Manalpha(1-3)Manbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
D-Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
3.2.1.114
GlcNAcbeta(1-2)Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, hydrolysis at equal rate as Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, galactosyl and sialyl-galactosyl derivatives are substrates, too
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
GlcNAcbeta(1-6)(GlcNAcbeta(1-2))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, rate at 50% of Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc hydrolysis
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced
586942
Aspergillus phoenicis
Manalpha(1-6)(Manalpha(1-3))D-Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced
586942
Aspergillus phoenicis
?
3.2.1.114
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
NaBH4 reduced, cleaves the Manalpha(1-6)Man linkage only after its Manalpha(1-3) residue is removed
586942
Aspergillus phoenicis
Manalpha(1-6)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
NaBH4 reduced, no product: Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
586942
Aspergillus phoenicis
?
3.2.1.114
additional information
not: 4-nitrophenyl alpha-mannoside
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
additional information
not: D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-4))[GlcNAcbeta(1-4)(GlcNAcbeta(1-2))Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, GlcNAcbeta(1-4)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc, all sodium borate reduced
586942
Aspergillus phoenicis
?
-
-
-
?
3.2.1.114
additional information
substrate specificity, hydrolysis mechanism of branched oligosaccharides
586942
Aspergillus phoenicis
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.114
37
-
assay at
Aspergillus phoenicis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.24
5
-
-
Aspergillus phoenicis
3.2.1.114
5
-
-
Aspergillus phoenicis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.114
3.5
6
pH 3.5: more than 70% of maximal activity, pH 6: about half-maximal activity
Aspergillus phoenicis