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Literature summary extracted from
- Isopropylmalate dehydratase from yeast (1988), Methods Enzymol., 166, 423-429.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.33 | - |
Saccharomyces cerevisiae |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.33 | half-life 2-3 h in potassium phosphate buffer, ph 6.8., 0-5°C, beta-isopropylmalate at 1 mM increases half-life to 15 h | Saccharomyces cerevisiae |
| 4.2.1.33 | stabilized by high concentrations of glycerol and ammonium sulfate which also inhibit activity, half-life with 50% glycerol, 2.07 M ammonium sulfate, and 30% glycerol plus 830 mM ammonium sulfate: 50 h, 57 h, and 90 h, respectively, instead of 2-3 h without, stabilization probably due to changes in intramolecular interactions | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.33 | KCN | complete inactivation at 10 mM; inactivation prevented by ammonium sulfate | Saccharomyces cerevisiae |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.33 | 0.048 | - |
dimethylcitraconate | crude enzyme preparation | Saccharomyces cerevisiae | |
| 4.2.1.33 | 0.083 | - |
citraconate | crude enzyme preparation | Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.33 | cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.33 | 90000 | - |
sucrose density gradient centrifugation, gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.33 | 2-isopropylmaleate + H2O | Saccharomyces cerevisiae | second of three reactions of leucine biosynthesis | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.33 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.33 | - |
Saccharomyces cerevisiae |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.2.1.33 | (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H2O | transcriptional feedback regulation by leucine | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.33 | 6.18 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.33 | 2-isopropylmaleate + H2O | second of three reactions of leucine biosynthesis | Saccharomyces cerevisiae | ? | - |
? | |
| 4.2.1.33 | citraconate | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 4.2.1.33 | dimethylcitraconate | - |
Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.33 | 30 | - |
enzyme assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.33 | 7 | 9 | - |
Saccharomyces cerevisiae |
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