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Literature summary extracted from

  • Reichenbecher Jr, V.E.; Gross, S.R.
    Structural features of normal and complemented forms of the Neurospora isopropylmalate isomerase (1978), J. Bacteriol., 133, 802-810.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.33 additional information
-
 complementation pair enzymes have higher molecular weights of 169000-185000 Neurospora crassa
4.2.1.33 88500
-
 sucrose density gradient centrifugation, gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.33 2-isopropylmaleate + H2O Neurospora crassa second of three reactions of leucine biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.33 Neurospora crassa
-
 wild type and complementing Leu-2 mutants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.33
-
 Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.33 additional information
-
-
 Neurospora crassa
4.2.1.33 54000
-
-
 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
4.2.1.33 -20°C, sodium phosphate buffer, pH 7.2, 6 M urea, several days Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.33 2-isopropylmaleate + H2O second of three reactions of leucine biosynthesis Neurospora crassa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.33 More amino acid analysis, protein contains hinge regions that are very sensitive to proteolytic attack Neurospora crassa
4.2.1.33 multimer active as monomer, can aggregate to dimers or multimers in cell Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.33 34
-
 enzyme assay at Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.33 6
-
 enzyme assay at Neurospora crassa