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Literature summary extracted from

  • Ueda, M.; Asano, Y.; Yamada, H.
    Purification and characterization of maleate hydratase from Arthrobacter sp. strain MC12612 (1993), Biosci. Biotechnol. Biochem., 57, 1545-1548.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.31 additional information activation by Fe2+ and L-cysteine, or other sulfhydryl compounds Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.31 2,2'-dipyridyl
-
 Arthrobacter sp.
4.2.1.31 CdCl2 24% inhibition at 2 mM Arthrobacter sp.
4.2.1.31 CuSO4 32% inhibition at 2 mM Arthrobacter sp.
4.2.1.31 Fe2(SO4)3 41% inhibition at 2 mM Arthrobacter sp.
4.2.1.31 HgCl2 25% inhibition at 2 mM Arthrobacter sp.
4.2.1.31 additional information
-
 Arthrobacter sp.
4.2.1.31 o-phenanthroline
-
 Arthrobacter sp.
4.2.1.31 p-chloromercuribenzoate
-
 Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.31 3.85
-
 Maleate
-
 Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.31 Fe2+ 353% activation at 2 mM Arthrobacter sp.
4.2.1.31 Mn2+ 49% activation at 2 mM Arthrobacter sp.
4.2.1.31 additional information
-
 Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.31 Arthrobacter sp.
-
-
-
4.2.1.31 Arthrobacter sp. MCI2612
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.31
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.31 6.55
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.31 maleate + H2O
-
 Arthrobacter sp. (R)-malate
-
 r
4.2.1.31 maleate + H2O
-
 Arthrobacter sp. MCI2612 (R)-malate
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.31 40
-
 20% inactivation after 30 min Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.31 8.5
-
-
 Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.31 7 8.5
-
 Arthrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.31 6
-
 rapidly inactivated below Arthrobacter sp.