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Literature summary extracted from
- Purification of tryptophan synthase from Saccharomyces cerevisiae and partial activity of its nicked subunits (1979), Eur. J. Biochem., 102, 167-172.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.20 | 0.07 | - |
indole | cosubstrate L-Ser | Saccharomyces cerevisiae |  |
| 4.2.1.20 | 0.17 | - |
indole | cosubstrate D-glyceraldehyde 3-phosphate | Saccharomyces cerevisiae | |
| 4.2.1.20 | 1.1 | - |
D-glyceraldehyde 3-phosphate | cosubstrate L-Trp, i.e. reverse reaction | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 77000 | - |
2 * 77000, sedimentation equilibrium in 6 M guanidine hydrochloride | Saccharomyces cerevisiae |
| 4.2.1.20 | 153000 | - |
sedimentation equilibrium centrifugation | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.20 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.20 | - |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.20 | -18°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.20 | 1-(indol-3-yl)glycerol 3-phosphate + L-serine | r | Saccharomyces cerevisiae | L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
| 4.2.1.20 | indole + D-glyceraldehyde 3-phosphate | r | Saccharomyces cerevisiae | indole-3-glycerol phosphate | r | ? | |
| 4.2.1.20 | indole + L-serine | - |
Saccharomyces cerevisiae | L-tryptophan + H2O | - |
? | |
| 4.2.1.20 | indole-3-glycerol phosphate | r | Saccharomyces cerevisiae | indole + D-glyceraldehyde 3-phosphate | r | ? | |
| 4.2.1.20 | L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | r | Saccharomyces cerevisiae | indole-3-glycerol phosphate + L-serine | r | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.20 | dimer | 2 * 77000, sedimentation equilibrium in 6 M guanidine hydrochloride | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.20 | 7.2 | - |
- |
Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.20 | pyridoxal 5'-phosphate | Km: 0.18 mM | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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