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Literature summary extracted from

  • Brand, N.J.; Fersht, A.R.
    Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus (1986), Gene, 44, 139-142.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.9 expression in Escherichia coli Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.9 0.023
-
valine enzyme expressed on E. coli Geobacillus stearothermophilus
6.1.1.9 0.024
-
valine
-
Geobacillus stearothermophilus
6.1.1.9 0.102
-
ATP
-
Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal
-
Geobacillus stearothermophilus AMP + diphosphate + L-valyl-tRNAVal
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.9 2.9
-
valine
-
Geobacillus stearothermophilus
6.1.1.9 5.65
-
valine enzyme expressed in E. coli Geobacillus stearothermophilus