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Literature summary extracted from

  • Armstrong, J.B.; Fairfield, J.A.
    A new method for the isolation of methionyl transfer synthetase mutants from Escherichia coli (1975), Can. J. Microbiol., 21, 754-758.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.10 additional information mutant strains with an increased Km for methionine Escherichia coli

General Stability

EC Number General Stability Organism
6.1.1.10 several cycles of freezing and thawing, cause no loss of activity of wild-type enzyme in crude extract Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.078
-
 Met wild-type enzyme Escherichia coli
6.1.1.10 0.48 1.63 Met Km-values of various mutant strains Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Escherichia coli
-
 and mutant strains with an increased Km for methionine
-

Storage Stability

EC Number Storage Stability Organism
6.1.1.10 -20°C, about 35% loss of activity after 6 months, wild-type enzyme in crude extract Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
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