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Literature summary extracted from

  • Narasimhan, C.; Miziorko, H.M.
    Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase: characterization of the isolated recombinant protein and investigation of the enzyme's cation requirements (1992), Biochemistry, 31, 11224-11230.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.3.4 extremely unstable, even in presence of glycerol Pseudomonas mevalonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.4 EDTA not Pseudomonas mevalonii
4.1.3.4 o-phenanthroline not m-Phenanthroline, probably acts as metal chelator Pseudomonas mevalonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.4 0.02
-
 hydroxymethylglutaryl-CoA wild-type Pseudomonas mevalonii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.4 Bivalent cations stimulate Pseudomonas mevalonii
4.1.3.4 Cu2+ tightly bound, stoichiometry Pseudomonas mevalonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.4 32000
-
 x * 32000, SDS-PAGE Pseudomonas mevalonii

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Pseudomonas mevalonii
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.4 overexpressed in Escherichia coli, immunoreaction with avian liver enzyme antibodies Pseudomonas mevalonii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.4 70
-
-
 Pseudomonas mevalonii

Storage Stability

EC Number Storage Stability Organism
4.1.3.4 -80°C, phosphate buffer, pH 7.2, 20% glycerol, several weeks Pseudomonas mevalonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 3-hydroxy-3-methylglutaryl-CoA
-
 Pseudomonas mevalonii acetoacetate + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.4 ? x * 32000, SDS-PAGE Pseudomonas mevalonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.4 30
-
 enzyme assay at Pseudomonas mevalonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.4 8.2
-
 enzyme assay at Pseudomonas mevalonii