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Literature summary extracted from

  • Chueskul, S.; Chulavatnatol, M.
    Properties of alpha-hydroxynitrile lyase from the petiole of cassava (Manihot esculenta Crantz) (1996), Arch. Biochem. Biophys., 334, 401-405.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.47 2-methyl-2-butanol
-
 Manihot esculenta
4.1.2.47 2-Methylbutyraldehyde
-
 Manihot esculenta
4.1.2.47 acetaldehyde
-
 Manihot esculenta
4.1.2.47 acetone
-
 Manihot esculenta
4.1.2.47 benzaldehyde
-
 Manihot esculenta
4.1.2.47 butanol
-
 Manihot esculenta
4.1.2.47 Chlorobutanol
-
 Manihot esculenta
4.1.2.47 ethanol
-
 Manihot esculenta
4.1.2.47 formaldehyde
-
 Manihot esculenta
4.1.2.47 isobutanol
-
 Manihot esculenta
4.1.2.47 Isobutyraldehyde
-
 Manihot esculenta
4.1.2.47 methanol
-
 Manihot esculenta
4.1.2.47 methyl ethyl ketone
-
 Manihot esculenta
4.1.2.47 Octanol
-
 Manihot esculenta
4.1.2.47 Pentanol
-
 Manihot esculenta
4.1.2.47 phenol
-
 Manihot esculenta
4.1.2.47 Propanol
-
 Manihot esculenta
4.1.2.47 propionaldehyde
-
 Manihot esculenta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.47 4
-
 acetone cyanohydrin
-
 Manihot esculenta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.47 25600
-
 4 * 25600, SDS-PAGE Manihot esculenta
4.1.2.47 102000
-
 gel filtration Manihot esculenta

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Manihot esculenta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.47 recombinant enzyme Manihot esculenta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.47 petiole
-
 Manihot esculenta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.47 47.5
-
-
 Manihot esculenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 2-hydroxy-2-methylpropanenitrile
-
 Manihot esculenta acetone + HCN
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.47 tetramer 4 * 25600, SDS-PAGE Manihot esculenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.47 5
-
-
 Manihot esculenta