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Literature summary extracted from

  • Large, P.J.
    Non-oxidative demethylation of trimethylamine N-oxide by Pseudomonas aminovorans (1979), FEBS Lett., 18, 297-300.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.2.32 enzyme is unstable when purified more than about 5-fold Aminobacter aminovorans
4.1.2.32 exposure of the enzyme for 30 min at 0°C to buffers of pH values in the region 4 to 9 does not result in loss of activity followed by adjustment of the pH 6.0 Aminobacter aminovorans
4.1.2.32 trimethylamine-grown Pseudomonas aminovorans enzyme is more labile at 40°C than enzyme from methylamine-grown bacteria in French press extracts Aminobacter aminovorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.32 cyanide active at 0.001 mM Aminobacter aminovorans
4.1.2.32 Mercurials active at 0.001 mM Aminobacter aminovorans
4.1.2.32 Triethylamine N-oxide
-
 Aminobacter aminovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.32 2
-
 Trimethylamine N-oxide
-
 Aminobacter aminovorans
4.1.2.32 140
-
 Benzyldimethylamine N-oxide
-
 Aminobacter aminovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.32 additional information Aminobacter aminovorans enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.32 Aminobacter aminovorans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.32 partial Aminobacter aminovorans

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.2.32 trimethylamine N-oxide = dimethylamine + formaldehyde non-oxidative, non-hydrolytic cleavage Aminobacter aminovorans
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.32 Benzyldimethylamine N-oxide 50% of the activity with trimethylamine N-oxide Aminobacter aminovorans ?
-
 ?
4.1.2.32 additional information enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium Aminobacter aminovorans ?
-
 ?
4.1.2.32 Trimethylamine N-oxide
-
 Aminobacter aminovorans Dimethylamine + formaldehyde
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.32 25
-
 assay at Aminobacter aminovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.32 6
-
-
 Aminobacter aminovorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.32 5 7.5 pH 5.0: about 75% of maximal activity, pH 7.5: about 75% of maximal activity Aminobacter aminovorans