BRENDA - Enzyme Database

Characterization of the glutamyl-tRNAGln-to-glutaminyl-tRNAGln amidotransferase reaction of Bacillus subtilis

Strauch, M.A.; Zalkin, H.; Aronson, A.I.; J. Bacteriol. 170, 916-920 (1988)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.7
L-methionine-S-sulfoximine
at 1 mM, 3 mM or 5 mM, 20% inhibition
Bacillus subtilis
6.3.5.7
additional information
no inhibition by 6-diazo-5-oxonorleucine
Bacillus subtilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.01
-
Gln
-
Bacillus subtilis
6.3.5.7
0.1
0.2
Asn
-
Bacillus subtilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.7
Bacillus subtilis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + Asn
Asn is much less effective as amide donor than glutamine
492310
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + Asp
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
492310
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + NH4Cl
NH4Cl is much less effective as amide donor than glutamine
492310
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + ?
-
-
-
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.5.7
L-methionine-S-sulfoximine
at 1 mM, 3 mM or 5 mM, 20% inhibition
Bacillus subtilis
6.3.5.7
additional information
no inhibition by 6-diazo-5-oxonorleucine
Bacillus subtilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.7
0.01
-
Gln
-
Bacillus subtilis
6.3.5.7
0.1
0.2
Asn
-
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.7
ATP + Glu-tRNAGln + Asn
Asn is much less effective as amide donor than glutamine
492310
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + Asp
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
492310
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + NH4Cl
NH4Cl is much less effective as amide donor than glutamine
492310
Bacillus subtilis
ADP + phosphate + Gln-tRNAGln + ?
-
-
-
?