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Literature summary extracted from

  • Daruwala, R.; Bhattacharyya, D.K.; Kwon, O.; Meganathan, R.
    Menaquinone (vitamin K2) biosynthesis: overexpression, purification, and characterization of a new isochorismate synthase from Escherichia coli (1997), J. Bacteriol., 179, 3133-3138.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.4.2 2-mercaptoethanol stimulates, 2.25fold stimulation at 20-30 mM Escherichia coli
5.4.4.2 dithiothreitol stimulates, 2fold stimulation at 1 mM Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.4.2 Cu2+
-
 Escherichia coli
5.4.4.2 Hg2+
-
 Escherichia coli
5.4.4.2 K+
-
 Escherichia coli
5.4.4.2 Mg2+ above 1 mM Escherichia coli
5.4.4.2 NEM
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.4.2 0.195
-
 chorismate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.4.2 Mg2+ sharp increase in activity, optimal concentration: 1 mM, inhibition above 1 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.4.2 48000
-
 2 * 48000, isochorismate synthase involved in enterobactin biosynthesis, MenF, SDS-PAGE Escherichia coli
5.4.4.2 98000
-
 isochorismate synthase involved in enterobactin biosynthesis, MenF, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.4.2 Chorismate Escherichia coli first committed step in the biosynthesis of menaquinone ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.4.2 Escherichia coli
-
 isochorismate synthase involved in enterobactin biosynthesis, MenF. The isochorismate synthase involved in menaquinone biosynthesis is different from the isochorismate synthase involved in enterobactin biosynthesis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.4.2
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.4.2 0.808
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
5.4.4.2 -20°C, 40% loss of activity after 5 days. 1% bovine serum albumin, 10% glycerol, or 20% dimethyl sulfoxide stabilize for up to 10 days Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.4.2 Chorismate not reversible, isochorismate synthase involved in enterobactin biosynthesis, MenF Escherichia coli Isochorismate
-
 ?
5.4.4.2 Chorismate first committed step in the biosynthesis of menaquinone Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.4.2 dimer 2 * 48000, isochorismate synthase involved in enterobactin biosynthesis, MenF, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.4.2 37
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.4.2 15 50 15°C: 72% of maximal activity, 20°C: about 74% of maximal activity, 30°C or 40°C: about 80% of maximal activity, 50°C: 42% of maximal activity Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.4.2 1.33
-
 chorismate in absence of 2-mercaptoethanol Escherichia coli
5.4.4.2 2.93
-
 chorismate in presence of 2-mercaptoethanol Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.4.2 6.5 9 about 20% of maximal activity at pH 6.5 and pH 9.0 Escherichia coli
5.4.4.2 7.5 8
-
 Escherichia coli