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Literature summary extracted from

  • Toyomasu, T.; Kawaide, H.; Ishizaki, A.; Shinoda, S.; Otsuka, M.; Mitsuhashi, W.; Sassa, T.
    Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional diterpene cyclase (2000), Biosci. Biotechnol. Biochem., 64, 660-664.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.19 expressed in Escherichia coli JM109, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity Fusarium fujikuroi
5.5.1.13 expression in Escherichia coli of a recombinant GST fused enzyme Fusarium fujikuroi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.19 107000
-
 predicted from cDNA sequence Fusarium fujikuroi
5.5.1.13 107000
-
 calculated from amino acid sequence Fusarium fujikuroi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Fusarium fujikuroi involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?
5.5.1.13 copalyl diphosphate Fusarium fujikuroi bifunctional enzyme ent-kaurene + diphosphate
-
 ?
5.5.1.13 geranylgeranyl diphosphate Fusarium fujikuroi gibberellin biosynthesis copalyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Fusarium fujikuroi Q9UVY5
-
-
5.5.1.13 Fusarium fujikuroi Q9UVY5 from strain IFO30336 mycelia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.13 recombinant enzyme, using Sepharose 4B glutathione affinity resin Fusarium fujikuroi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
 Fusarium fujikuroi ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Fusarium fujikuroi ent-kaurene + diphosphate
-
 ?
5.5.1.13 copalyl diphosphate bifunctional enzyme Fusarium fujikuroi ent-kaurene + diphosphate
-
 ?
5.5.1.13 geranylgeranyl diphosphate
-
 Fusarium fujikuroi copalyl diphosphate
-
 ?
5.5.1.13 geranylgeranyl diphosphate gibberellin biosynthesis Fusarium fujikuroi copalyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.19 tetramer 4 * 107000 Fusarium fujikuroi

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Fusarium fujikuroi