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Literature summary extracted from

  • Schneider, K.; Kastner, C.N.; Meyer, M.; Wessel, M.; Dimroth, P.; Bott, M.
    Identification of a gene cluster in Klebsiella pneumoniae which includes citX, a gene required for biosynthesis of the citrate lyase prosthetic group (2002), J. Bacteriol., 184, 2439-2446.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.61 expressed in Escherichia coli BL21-DE3 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.6 Mg2+ dependent Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.61 20100
-
 calculated from the amino acids number Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.6 citrate Klebsiella pneumoniae
-
 acetate + oxaloacetate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.52 Klebsiella pneumoniae P45414
-
-
2.7.7.61 Klebsiella pneumoniae
-
 wild type
-
2.7.7.61 Klebsiella pneumoniae Q8VS40 strain ATCC 13882
-
4.1.3.6 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.61
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.61 additional information
-
 maximal CitX content is present in cells grown anaerobically with citrate as the sole carbon and energy source Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.6 citrate
-
 Klebsiella pneumoniae acetate + oxaloacetate
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.3.6 2'-(5''-phosphoribosyl)-3'-dephospho-coenzyme A CitG and CitX catalyze the initial formation of the precursor 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA and subsequent transfer to apo-citrate lyase with removal of diphosphate Klebsiella pneumoniae

Expression

EC Number Organism Comment Expression
2.4.2.52 Klebsiella pneumoniae enzyme is part of the citrate lyase gene cluster, citCDEFXG additional information