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Literature summary extracted from

  • Pohlig, G.; Wingender-Drissen, R.; Becker, J.U.
    Characterization of phosphorylase kinase activities in yeast (1983), Biochem. Biophys. Res. Commun., 114, 331-338.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.19 5%, w/v, glycerol stabilizes Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 Mg2+ not Saccharomyces cerevisiae
2.7.11.19 additional information no inhibition by cAMP-binding protein Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 0.018
-
 ATP
-
 Saccharomyces cerevisiae
2.7.11.19 0.6
-
 GTP
-
 Saccharomyces cerevisiae
2.7.11.19 1.4
-
 UTP
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.19 cytosol
-
 Saccharomyces cerevisiae 5829
-
2.7.11.19 soluble
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Mg2+ requirement Saccharomyces cerevisiae
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Saccharomyces cerevisiae
2.7.11.19 phosphate requirement, phosphate containing enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 29000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Saccharomyces cerevisiae
-
 commercial baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + glycogen synthase at high concentration, from yeast Saccharomyces cerevisiae ADP + phosphoglycogen synthase
-
 ?
2.7.11.19 ATP + phosphorylase b yeast Saccharomyces cerevisiae ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Saccharomyces cerevisiae ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b rabbit skeletal muscle Saccharomyces cerevisiae ADP + phosphorylase a
-
 ?
2.7.11.19 GTP + phosphorylase b cosubstrate: Mg-UTP complex Saccharomyces cerevisiae GDP + phosphorylase a
-
 ?
2.7.11.19 UTP + phosphorylase b cosubstrate: Mg-UTP complex Saccharomyces cerevisiae UDP + phosphorylase a
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 8
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.19 7.2 9 70% of maximal activity at pH 7.2 and and 9 Saccharomyces cerevisiae