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Literature summary extracted from

  • Cooper, R.H.; Sul, H.S.; McCullough, T.E.; Walsh, D.
    Purification and properties of the cardiac isoenzyme of phosphorylase kinase (1980), J. Biol. Chem., 255, 11794-11801.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 adenosine 3',5'-monophosphate
-
 Bos taurus
2.7.11.19 Catalytic subunit of cAMP-dependent protein kinase activation of nonactivated enzyme Bos taurus
2.7.11.19 Catalytic subunit of cAMP-dependent protein kinase by phosphorylation of alpha' Bos taurus
2.7.11.19 Catalytic subunit of cAMP-dependent protein kinase and beta (not gamma) Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 ATP otherwise activating; total inhibition if ATP concentration exceeds that of divalent cation Bos taurus
2.7.11.19 EGTA Ca2+ restores; strong Bos taurus
2.7.11.19 GTP weak, with ATP as substrate Bos taurus
2.7.11.19 ITP weak, with ATP as substrate Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 0.14 0.22 ATP at different phosphorylase b concentrations Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.19 cytosol
-
 Bos taurus 5829
-
2.7.11.19 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ca2+ requirement Bos taurus
2.7.11.19 Mg2+ requirement Bos taurus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Bos taurus
2.7.11.19 Mn2+ requirement Bos taurus
2.7.11.19 Mn2+ can substitute for Mg2+ (less effective) Bos taurus
2.7.11.19 phosphate requirement, phosphate containing enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 48000
-
 4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin Bos taurus
2.7.11.19 125000
-
 4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin Bos taurus
2.7.11.19 134000
-
 4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin Bos taurus
2.7.11.19 1300000
-
 bovine, sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 to near homogeneity Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 cardiac muscle
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 3.68
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.19 -70°C, several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Bos taurus ADP + phosphorylase a
-
 ?
2.7.11.19 additional information ITP, and CTP are no substrates Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.19 hexadecamer 4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 8.8
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.19 6.2 8.8 progressive increase of activity Bos taurus