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Literature summary extracted from

  • Gallagher, P.J.; Herring, B.P.; Griffin, S.A.; Stull, J.T.
    Molecular characterization of a mammalian smooth muscle myosin light chain kinase [published erratum appears in J Biol Chem 1992 May 5;267(13):9450] (1991), J. Biol. Chem., 266, 23936-23944.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 expression in COS cells Oryctolagus cuniculus
2.7.11.18 expression in COS-cells Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.005 0.0095 myosin light chain
-
 Gallus gallus
2.7.11.18 0.005 0.0095 myosin light chain
-
 Bos taurus
2.7.11.18 0.005 0.0095 myosin light chain
-
 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+
-
 Gallus gallus
2.7.11.18 Ca2+
-
 Rattus norvegicus
2.7.11.18 Ca2+
-
 Bos taurus
2.7.11.18 Ca2+
-
 Oryctolagus cuniculus
2.7.11.18 Ca2+ Ca2+/calmodulin-dependent Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 125700
-
 calculated from sequence of DNA Oryctolagus cuniculus
2.7.11.18 136000
-
 x * 136000, SDS-PAGE, enzyme from smooth muscle Rattus norvegicus
2.7.11.18 152000
-
 x * 152000, SDS-PAGE, recombinant enzyme Oryctolagus cuniculus
2.7.11.18 155000
-
 x * 155000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Oryctolagus cuniculus
-
 ADP + phosphorylated myosin light chain
-
 ?
2.7.11.18 ATP + myosin light chain Rattus norvegicus
-
 ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain Gallus gallus event in initiation of smooth-muscle contraction ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain Bos taurus event in initiation of smooth-muscle contraction ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain Oryctolagus cuniculus event in initiation of smooth-muscle contraction ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Bos taurus
-
-
-
2.7.11.18 Gallus gallus
-
-
-
2.7.11.18 Oryctolagus cuniculus
-
-
-
2.7.11.18 Oryctolagus cuniculus P29294
-
-
2.7.11.18 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 adrenal medulla not rat Gallus gallus
-
2.7.11.18 adrenal medulla not rat Bos taurus
-
2.7.11.18 adrenal medulla not rat Oryctolagus cuniculus
-
2.7.11.18 aorta
-
 Bos taurus
-
2.7.11.18 kidney not rat Gallus gallus
-
2.7.11.18 kidney not rat Bos taurus
-
2.7.11.18 kidney not rat Oryctolagus cuniculus
-
2.7.11.18 liver not rat Gallus gallus
-
2.7.11.18 liver not rat Bos taurus
-
2.7.11.18 liver not rat Oryctolagus cuniculus
-
2.7.11.18 additional information myosin light chain kinases in smooth muscle and non-muscle tissues are the same protein Gallus gallus
-
2.7.11.18 additional information myosin light chain kinases in smooth muscle and non-muscle tissues are the same protein Rattus norvegicus
-
2.7.11.18 additional information myosin light chain kinases in smooth muscle and non-muscle tissues are the same protein Bos taurus
-
2.7.11.18 additional information myosin light chain kinases in smooth muscle and non-muscle tissues are the same protein Oryctolagus cuniculus
-
2.7.11.18 muscle
-
 Oryctolagus cuniculus
-
2.7.11.18 smooth muscle
-
 Rattus norvegicus
-
2.7.11.18 smooth muscle
-
 Bos taurus
-
2.7.11.18 smooth muscle uterus, trachea, aorta, ileum, gizzard Gallus gallus
-
2.7.11.18 smooth muscle uterus, trachea, aorta, ileum, gizzard Oryctolagus cuniculus
-
2.7.11.18 uterus
-
 Oryctolagus cuniculus
-
2.7.11.18 uterus not rat Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + myosin light chain
-
 Oryctolagus cuniculus ADP + phosphorylated myosin light chain
-
 ?
2.7.11.18 ATP + myosin light chain
-
 Rattus norvegicus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Gallus gallus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Rattus norvegicus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain acceptor substrates are myosin light chains of smooth muscle Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain event in initiation of smooth-muscle contraction Gallus gallus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain event in initiation of smooth-muscle contraction Bos taurus ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain event in initiation of smooth-muscle contraction Oryctolagus cuniculus ADP + myosin light chain phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 ?
-
 Gallus gallus
2.7.11.18 ? x * 136000, SDS-PAGE, enzyme from smooth muscle Rattus norvegicus
2.7.11.18 ? x * 152000, SDS-PAGE, recombinant enzyme Oryctolagus cuniculus
2.7.11.18 ? x * 155000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 myosin light chain kinase, smooth muscle
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin requirement Gallus gallus
2.7.11.18 Calmodulin requirement Rattus norvegicus
2.7.11.18 Calmodulin requirement Bos taurus
2.7.11.18 Calmodulin requirement Oryctolagus cuniculus