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Literature summary extracted from

  • Kline, L.K.; Fittler, F.; Hall, R.H.
    N6-(delta-2-isopentenyl) adenosine. Biosynthesis in transfer ribonucleic acid in vitro (1969), Biochemistry, 8, 4361-4371.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.75 diphosphate
-
 Saccharomyces cerevisiae
2.5.1.75 EDTA
-
 Saccharomyces cerevisiae
2.5.1.75 iodoacetamide
-
 Saccharomyces cerevisiae
2.5.1.75 p-mercuribenzoate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.75 Mg2+ required Saccharomyces cerevisiae
2.5.1.75 Mg2+ optimal concentration: 5 mM Saccharomyces cerevisiae
2.5.1.75 Mn2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA Saccharomyces cerevisiae biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.75 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.75 partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.75 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA specific for DELTA2-isopentenyl phosphate Saccharomyces cerevisiae diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA no reaction with DELTA3-isopentenyl diphosphate Saccharomyces cerevisiae diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA no reaction with homologous native tRNA or homologous, permanganate-treated tRNA (permanganate specifically cleaves the DELTA2-isopentenyl groups of tRNA leaving adenosine residues) Saccharomyces cerevisiae diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA the enzyme from both yeast and rat liver catalyzes a significant incorporation of DELTA2-isopentenyl groups into untreated E. coli B tRNA Saccharomyces cerevisiae diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon Saccharomyces cerevisiae diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.75 7.5 8
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.75 7 9.5 pH 7: about 95% of maximum activity, pH 9.5: about 45% of maximum activity Saccharomyces cerevisiae