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Literature summary extracted from

  • Williams, D.C.; Morgan, G.S.; McDonald, E.; Battersby, A.R.
    Purification of porphobilinogen deaminase from Euglena gracilis and studies of its kinetics (1981), Biochem. J., 193, 301-310.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 41000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 additional information Euglena gracilis in presence of a second enzyme, EC 4.2.1.75, uroporphyrinogen-III synthase, often called co-synthase, the product is cyclized to form uroporphyrinogen-III ?
-
 ?
2.5.1.61 porphobilinogen Euglena gracilis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 0.1
-
-
 Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
2.5.1.61 0-4°C, less than 5% loss of activity over 10 days Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Euglena gracilis uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O stoichiometry of enzyme reaction Euglena gracilis hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 additional information in presence of a second enzyme, EC 4.2.1.75, uroporphyrinogen-III synthase, often called co-synthase, the product is cyclized to form uroporphyrinogen-III Euglena gracilis ?
-
 ?
2.5.1.61 porphobilinogen
-
 Euglena gracilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 porphobilinogen deaminase
-
 Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 additional information
-
 stoichiometry and kinetic mechanism of the enzyme reaction at different pH values Euglena gracilis
2.5.1.61 6 9 pH 6.0: 40% of maximal activity, pH 9.0: 35% of maximal activity Euglena gracilis