Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Anderson, P.M.; Desnick, R.J.
    Purification and properties of uroporphyrinogen I synthase from human erythrocytes (1980), J. Biol. Chem., 255, 1993-1999.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.61 Ca2+ 0.1 mM CaCl2, 80% inhibition Homo sapiens
2.5.1.61 Cu2+ 1 mM CuSO4, 87% inhibition Homo sapiens
2.5.1.61 Fe2+ 1 mM FeSO4, 58% inhibition Homo sapiens
2.5.1.61 Fe3+ 1 mM FeCl3, 62% inhibition Homo sapiens
2.5.1.61 Hg2+ 0.0004 mM HgCl2, 80% inhibition Homo sapiens
2.5.1.61 Mg2+ 50 mM MgCl2, 55% inhibition Homo sapiens
2.5.1.61 N-ethylmaleimide 1 mM 38% inhibition Homo sapiens
2.5.1.61 p-chloromercuribenzoate 0.002 mM, 74% inhibition Homo sapiens
2.5.1.61 Pb(NO3)2 0.005 mM, 35% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.006
-
 porphobilinogen A and B form Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 36000
-
 gel filtration, A form Homo sapiens
2.5.1.61 37000
-
 1 * 37000, SDS-PAGE, A and B form Homo sapiens
2.5.1.61 38000
-
 gel filtration, B form Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 additional information Homo sapiens occurrence of multiple forms of the enzyme. These isomers correspond to the enzyme-substrate intermediates: mono-, di-, tri-, and tetrapyrrole ?
-
 ?
2.5.1.61 porphobilinogen Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61 5 forms, A: native enzyme, B-E: isomeres corresponding to the enzyme-substrate intermediates Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 additional information
-
 enzyme activity is temperature-dependent Homo sapiens
2.5.1.61 0.038
-
 A form Homo sapiens
2.5.1.61 0.039
-
 B form Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.5.1.61 -20°C, forms A to E, stable for up to 18 months in 10 mM potassium phosphate buffer, pH 8.0, containing 0.2 mM dithioerythritol Homo sapiens
2.5.1.61 erythrocytes lysates stored in 1 mM potassium phosphate, pH 7.6, containing 0.05% Trition X-100, 1 mM dithiothreitol and 1 mM MgCl2, no or little loss of activity after 1 year Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Homo sapiens uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Homo sapiens hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 additional information occurrence of multiple forms of the enzyme. These isomers correspond to the enzyme-substrate intermediates: mono-, di-, tri-, and tetrapyrrole Homo sapiens ?
-
 ?
2.5.1.61 porphobilinogen
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.61 monomer 1 * 37000, SDS-PAGE, A and B form Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 uroporphyrinogen I synthase
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.61 additional information
-
-
 Homo sapiens
2.5.1.61 56
-
 2h, no loss of activity Homo sapiens
2.5.1.61 60
-
 2 h, 10% loss of activity Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.61 0.25
-
 porphobilinogen
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 8.2
-
 A and B form Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 6 9 pH 6.0: no activity below, pH 9.0: 50% of maximal activity Homo sapiens