Literature summary extracted from

Tsuda, M.; Katunuma, N.; Weber, G.
Rat liver glutamine 5-phosphoribosyl-1-pyrophosphate amidotransferase [EC 2.4.2.14]. Purification and properties (1979), J. Biochem., 85, 1347-1354.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.4.2.14	AMP	no inhibition in the presence of 5-phospho-alpha-D-ribose 1-diphosphate at high concentrations	Rattus norvegicus
2.4.2.14	GMP	no inhibition in the presence of 5-phospho-alpha-D-ribose 1-diphosphate at high concentrations	Rattus norvegicus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.4.2.14	1.24	1.5	L-glutamine	-	Rattus norvegicus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
2.4.2.14	soluble	-	Rattus norvegicus	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.2.14	195000	-	sucrose density gradient centrifugation	Rattus norvegicus
2.4.2.14	200000	-	native PAGE	Rattus norvegicus
2.4.2.14	215000	-	gel filtration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.2.14	L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O	Rattus norvegicus	rate-limiting enzyme of purine biosynthesis	L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.2.14	Rattus norvegicus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.2.14	heat treatment, acid precipitation, ammonium sulfate, ethanol, Sephadex G-150, DE-52	Rattus norvegicus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.4.2.14	liver	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.4.2.14	1.82	-	-	Rattus norvegicus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.2.14	L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O	-	Rattus norvegicus	5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate	-	?
2.4.2.14	L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O	rate-limiting enzyme of purine biosynthesis	Rattus norvegicus	L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.4.2.14	37	-	assay at	Rattus norvegicus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.4.2.14	6.5	8.5	broad, Tris or phosphate buffer, constant activity	Rattus norvegicus

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Release 2023.1 (January 2023)