BRENDA - Enzyme Database show

Pyridine nucleotide synthesis. Purification of nicotinamide mononucleotide pyrophosphorylase from rat erythrocytes

Lin, L.F.H.; Lan, S.J.; Richardson, A.H.; Henderson, L.V.M.; J. Biol. Chem. 247, 8016-8022 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.12
EDTA
50% activation, 1 mM
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.4.2.12
bovine serum albumin, glutathione, cysteine, DTT, EDTA, cystine or H2O2 does not stabilize during storage
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.000067
-
nicotinamide
-
Rattus norvegicus
2.4.2.12
0.0038
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
requirement
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Rattus norvegicus
first step in NAD synthesis from nicotinamide
nicotinamide D-ribonucleotide + diphosphate
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.12
partial
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
erythrocyte
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
0.000225
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.4.2.12
-20°C to +20°C, about 70% loss of activity overnight
Rattus norvegicus
2.4.2.12
storage at room temperature, 4°C, or frozen, unstable upon
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
-
489738
Rattus norvegicus
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
first step in NAD synthesis from nicotinamide
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
-
489738
Rattus norvegicus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.12
8.5
9
assay at pH 8.8. where NAD glycohydrolase is not active
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.12
7.8
9.2
about half-maximal activity at pH 7.8 and about 80% of maximal activity at pH 9.2
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.12
7
10
stable
Rattus norvegicus
2.4.2.12
8.8
-
rapid inactivation below
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
requirement
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.12
EDTA
50% activation, 1 mM
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
requirement
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.12
bovine serum albumin, glutathione, cysteine, DTT, EDTA, cystine or H2O2 does not stabilize during storage
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.000067
-
nicotinamide
-
Rattus norvegicus
2.4.2.12
0.0038
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
requirement
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Rattus norvegicus
first step in NAD synthesis from nicotinamide
nicotinamide D-ribonucleotide + diphosphate
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
partial
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
erythrocyte
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
0.000225
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.2.12
-20°C to +20°C, about 70% loss of activity overnight
Rattus norvegicus
2.4.2.12
storage at room temperature, 4°C, or frozen, unstable upon
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
-
489738
Rattus norvegicus
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
first step in NAD synthesis from nicotinamide
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
-
489738
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.12
8.5
9
assay at pH 8.8. where NAD glycohydrolase is not active
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.12
7.8
9.2
about half-maximal activity at pH 7.8 and about 80% of maximal activity at pH 9.2
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.4.2.12
7
10
stable
Rattus norvegicus
2.4.2.12
8.8
-
rapid inactivation below
Rattus norvegicus