Data extracted from this reference:
Activating Compound
2.4.2.12
EDTA
50% activation, 1 mM
Rattus norvegicus
General Stability
2.4.2.12
bovine serum albumin, glutathione, cysteine, DTT, EDTA, cystine or H2O2 does not stabilize during storage
Rattus norvegicus
KM Value [mM]
2.4.2.12
0.000067
nicotinamide
Rattus norvegicus
2.4.2.12
0.0038
5-phospho-alpha-D-ribose 1-diphosphate
Rattus norvegicus
Localization
2.4.2.12
soluble
Rattus norvegicus
Metals/Ions
2.4.2.12
Mg2+
requirement
Rattus norvegicus
Natural Substrates/ Products (Substrates)
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Rattus norvegicus
first step in NAD synthesis from nicotinamide
nicotinamide D-ribonucleotide + diphosphate
Rattus norvegicus
?
Organism
2.4.2.12
Rattus norvegicus
Purification (Commentary)
2.4.2.12
partial
Rattus norvegicus
Source Tissue
2.4.2.12
erythrocyte
Rattus norvegicus
Specific Activity [micromol/min/mg]
2.4.2.12
0.000225
Rattus norvegicus
Storage Stability
2.4.2.12
-20°C to +20°C, about 70% loss of activity overnight
Rattus norvegicus
2.4.2.12
storage at room temperature, 4°C, or frozen, unstable upon
Rattus norvegicus
Substrates and Products (Substrate)
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
489738
Rattus norvegicus
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
first step in NAD synthesis from nicotinamide
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
489738
Rattus norvegicus
?
Temperature Optimum [°C]
2.4.2.12
37
assay at
Rattus norvegicus
pH Optimum
2.4.2.12
8.5
9
assay at pH 8.8. where NAD glycohydrolase is not active
Rattus norvegicus
pH Range
2.4.2.12
7.8
9.2
about half-maximal activity at pH 7.8 and about 80% of maximal activity at pH 9.2
Rattus norvegicus
pH Stability
2.4.2.12
7
10
stable
Rattus norvegicus
2.4.2.12
8.8
rapid inactivation below
Rattus norvegicus
Cofactor
2.4.2.12
ATP
requirement
Rattus norvegicus
Activating Compound (protein specific)
2.4.2.12
EDTA
50% activation, 1 mM
Rattus norvegicus
Cofactor (protein specific)
2.4.2.12
ATP
requirement
Rattus norvegicus
General Stability (protein specific)
2.4.2.12
bovine serum albumin, glutathione, cysteine, DTT, EDTA, cystine or H2O2 does not stabilize during storage
Rattus norvegicus
KM Value [mM] (protein specific)
2.4.2.12
0.000067
nicotinamide
Rattus norvegicus
2.4.2.12
0.0038
5-phospho-alpha-D-ribose 1-diphosphate
Rattus norvegicus
Localization (protein specific)
2.4.2.12
soluble
Rattus norvegicus
Metals/Ions (protein specific)
2.4.2.12
Mg2+
requirement
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Rattus norvegicus
first step in NAD synthesis from nicotinamide
nicotinamide D-ribonucleotide + diphosphate
Rattus norvegicus
?
Purification (Commentary) (protein specific)
2.4.2.12
partial
Rattus norvegicus
Source Tissue (protein specific)
2.4.2.12
erythrocyte
Rattus norvegicus
Specific Activity [micromol/min/mg] (protein specific)
2.4.2.12
0.000225
Rattus norvegicus
Storage Stability (protein specific)
2.4.2.12
-20°C to +20°C, about 70% loss of activity overnight
Rattus norvegicus
2.4.2.12
storage at room temperature, 4°C, or frozen, unstable upon
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
489738
Rattus norvegicus
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
first step in NAD synthesis from nicotinamide
489738
Rattus norvegicus
nicotinamide D-ribonucleotide + diphosphate
489738
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
2.4.2.12
37
assay at
Rattus norvegicus
pH Optimum (protein specific)
2.4.2.12
8.5
9
assay at pH 8.8. where NAD glycohydrolase is not active
Rattus norvegicus
pH Range (protein specific)
2.4.2.12
7.8
9.2
about half-maximal activity at pH 7.8 and about 80% of maximal activity at pH 9.2
Rattus norvegicus
pH Stability (protein specific)
2.4.2.12
7
10
stable
Rattus norvegicus
2.4.2.12
8.8
rapid inactivation below
Rattus norvegicus