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Literature summary extracted from

  • Ishikura, N.; Yang, Z.Q.
    Multiple forms of flavonol O-glucosyltransferases in young leaves of Euonymus alatus f. ciliato-denatus (1994), Phytochemistry, 36, 1139-1145.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.91 2-mercaptoethanol 14 mM, activation of isoenzyme F3GT1 to 160%, activation of isoenzyme F3GT2 to 136% Euonymus alatus
2.4.1.91 EDTA slight activation Euonymus alatus
2.4.1.237 2-mercaptoethanol 14 mM, stimulates Euonymus alatus
2.4.1.237 EDTA 1 mM: slight activation, 10 mM: 36% inhibition Euonymus alatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.91 CaCl2 10 mM, 9% inhibition Euonymus alatus
2.4.1.91 CoCl2 1 mM, 50% inhibition of isoenzyme F3GT1 and 25% inhibition of isoenzyme F3GT2 Euonymus alatus
2.4.1.91 Cu2+ 1 mM CuCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2 Euonymus alatus
2.4.1.91 dithioerythritol
-
 Euonymus alatus
2.4.1.91 EDTA 10 mM, slight inhibition Euonymus alatus
2.4.1.91 iodoacetamide
-
 Euonymus alatus
2.4.1.91 iodoacetate
-
 Euonymus alatus
2.4.1.91 KCl 10 mM, 9-10% inhibition Euonymus alatus
2.4.1.91 Mn2+ 10 mM MnCl2, 52% inhibition of isoenzyme F3GT1 and 28% inhibition of isoenzyme F3GT2 Euonymus alatus
2.4.1.91 NEM
-
 Euonymus alatus
2.4.1.91 PCMB
-
 Euonymus alatus
2.4.1.91 Phenylmercuriacetate
-
 Euonymus alatus
2.4.1.91 Zn2+ 1 mM ZnCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2 Euonymus alatus
2.4.1.237 Ca2+ 10 mM, 35% inhibition Euonymus alatus
2.4.1.237 Co2+ 1 mM, 32% inhibition Euonymus alatus
2.4.1.237 Cu2+ 1 mM, 94% inhibition Euonymus alatus
2.4.1.237 dithioerythritol 10 mM, 13% inhibition Euonymus alatus
2.4.1.237 EDTA 10 mM: 36% inhibition, 1 mM: slight activation Euonymus alatus
2.4.1.237 iodoacetamide 1 mM, 28% inhibition Euonymus alatus
2.4.1.237 iodoacetate 1 mM, 16% inhibition Euonymus alatus
2.4.1.237 KCl 1 or 10 mM, 31% inhibition Euonymus alatus
2.4.1.237 Mg2+ 10 mM, 20% inhibition Euonymus alatus
2.4.1.237 Mn2+ 10 mM, 37% inhibition Euonymus alatus
2.4.1.237 N-ethylmaleimide 1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity Euonymus alatus
2.4.1.237 p-chloromercuribenzoate 1 mM, 97% inhibition, 14 mM 2-mercaptoethanol partially restores activity Euonymus alatus
2.4.1.237 Phenylmercuriacetate 1 mM, 96% inhibition Euonymus alatus
2.4.1.237 Zn2+ 1 mM, 89% inhibition Euonymus alatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.237 0.002
-
 UDP-glucose pH 7.5, 30°C, cosubstrate kaempferol Euonymus alatus
2.4.1.237 0.0111
-
 kaempferol pH 7.5, 30°C Euonymus alatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.91 Mg2+ 10 mM, slight stimulatory effect on isoenzyme F3GT1 and F3GT2 Euonymus alatus
2.4.1.237 additional information no requirement for Mg2+ Euonymus alatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.91 48000
-
 gel filtration Euonymus alatus
2.4.1.237 48000
-
 gel filtration Euonymus alatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.237 additional information Euonymus alatus glycosylation of flavonols ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Euonymus alatus
-
 f. Ciliato-dentatus
-
2.4.1.237 Euonymus alatus
-
 f. Ciliato-dentatus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91 isoenzyme F3GT1 and F3GT2 Euonymus alatus
2.4.1.237 139.9fold, partial Euonymus alatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 leaf young Euonymus alatus
-
2.4.1.237 leaf young Euonymus alatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.237 1.76
-
 pH 7.5, 30°C, kaempferol as substrate Euonymus alatus

Storage Stability

EC Number Storage Stability Organism
2.4.1.237 -20°C, partially purified enzyme, 20 mM Tris-HCl, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, 2 days: 18% loss of activity, 8 days: 64% loss of activity Euonymus alatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 UDP-alpha-D-glucose + isorhamnetin
-
 Euonymus alatus UDP + isorhamnetin 3-O-beta-D-glucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + kaempferol 7-O-glucoside isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 5% of the activity with kaempferol Euonymus alatus UDP + kaempferol 3,7-O-diglucoside
-
 ?
2.4.1.91 UDP-alpha-D-glucose + quercetin 7-O-beta-D-glucoside isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 6% of the activity with kaempferol Euonymus alatus UDP + quercetin 3,7-O-di-beta-D-glucoside
-
 ?
2.4.1.91 UDPglucose + 3,5,7-trihydroxyflavanone isoenzyme F3GT1: 22% of the activity with kaempferol, isoenzyme F3GT2: 12% of the activity with kaempferol Euonymus alatus UDP + 3,5,7-trihydroxyflavanone 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + 7,4'-dihydroxyflavonol isoenzyme F3GT1: 33% of the activity with kaempferol, isoenzyme F3GT2: 34% of the activity with kaempferol Euonymus alatus UDP + 7,4'-dihydroxyflavonol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + dihydrokaempferol isoenzyme F3GT1: 7% of the activity with kaempferol, isoenzyme F3GT2: 7% of the activity with kaempferol Euonymus alatus UDP + dihydrokaempferol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + dihydroquercetin isoenzyme F3GT1: 3% of the activity with kaempferol, isoenzyme F3GT2: 2% of the activity with kaempferol Euonymus alatus UDP + dihydroquercetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + fisetin
-
 Euonymus alatus UDP + fisetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol
-
 Euonymus alatus UDP + kaempferol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol 5,7,4'-trimethyl ether isoenzyme F3GT1: 52% of the activity with kaempferol, isoenzyme F3GT2: 11% of the activity with kaempferol Euonymus alatus UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol-4'-O-methylether
-
 Euonymus alatus UDP + ?
-
 ?
2.4.1.91 UDPglucose + myricetin
-
 Euonymus alatus UDP + myrecetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin
-
 Euonymus alatus UDP + quercetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin 5-methyl ether isoenzyme F3GT1: 38% of the activity with kaempferol, isoenzyme F3GT2: 25% of the activity with kaempferol Euonymus alatus UDP + quercetin 5-methyl ether 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + rhamnetin isoenzyme F3GT1: 20% of the activity with kaempferol, isoenzyme F3GT2: 82% of the activity with kaempferol Euonymus alatus UDP + rhamnetin 3-O-glucoside
-
 ?
2.4.1.237 additional information glycosylation of flavonols Euonymus alatus ?
-
 ?
2.4.1.237 additional information no or very poor substrates: fisetin, kaempferol 5,7,4’-trimethylether, rhamnetin, kaempferol 7-O-glucoside, quercetin 7-O-glucoside, taxifolin, dihydrokaempferol, ADP-D-glucose, GDP-D-glucose Euonymus alatus ?
-
 ?
2.4.1.237 UDP-alpha-D-glucose + kaempferol good substrate Euonymus alatus UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-alpha-D-glucose + quercetin 29% of the activity with kaempferol as substrate Euonymus alatus UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 3,5,7-trihydroxyflavanone pinobanksin, 55% of the activity with kaempferol as substrate Euonymus alatus UDP + 3,5,7-trihydroxyflavanone 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + 7,4'-dihydroxyflavonol 53% of the activity with kaempferol as substrate Euonymus alatus UDP + 7,4'-dihydroxyflavonol 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + a flavonol
-
 Euonymus alatus UDP + a flavonol 7-O-beta-D-glucoside F7GT mediates the transfer of D-glucose exclusively to the 7-hydroxyl group of kaempferol and some other flavonols, highly specific for UDP-D-glucose as glucosyl donor ?
2.4.1.237 UDP-glucose + euonymin 16% of the activity with kaempferol as substrate Euonymus alatus UDP + euonymin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + isoquercitrin 13% of the activity with kaempferol as substrate Euonymus alatus UDP + isoquercitrin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + isorhamnetin 128% of the activity with kaempferol as substrate Euonymus alatus UDP + isorhamnetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferide 104% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferide 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 3,4'-di-O-glucoside 12% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferol 3,7,4'-tri-O-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 3,4'-dimethylether 31% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferol 3,4'-dimethylether 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + kaempferol 3-O-glucoside astragalin, 20% of the activity with kaempferol as substrate Euonymus alatus UDP + kaempferol 3,7-O-diglucoside
-
 ?
2.4.1.237 UDP-glucose + myricetin 36% of the activity with kaempferol as substrate Euonymus alatus UDP + myricetin 7-O-beta-D-glucoside
-
 ?
2.4.1.237 UDP-glucose + quercetin 5-methylether 13% of the activity with kaempferol as substrate Euonymus alatus UDP + quercetin 5-methylether 7-O-beta-D-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.237 monomer 1 * 48000, SDS-PAGE Euonymus alatus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.237 F7GT
-
 Euonymus alatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.237 30
-
 assay at Euonymus alatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 7.5
-
-
 Euonymus alatus
2.4.1.237 7.5
-
 Tris-HCl buffer Euonymus alatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.237 Euonymus alatus chromatofocusing
-
 4.5