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Literature summary extracted from

  • Christner, J.E.; Distler, J.J.; Jourdian, G.W.
    Biosynthesis of keratan sulfate: purification and properties of a galactosyltransferase from bovine cornea (1979), Arch. Biochem. Biophys., 192, 548-558.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.38 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Bos taurus
2.4.1.90 alpha-lactalbumin stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.38 alpha-lactalbumin inhibits reaction with UDPgalactose and N-acetylglucosamine Bos taurus
2.4.1.90 alpha-lactalbumin inhibits reaction with UDPgalactose and N-acetylglucosamine Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.38 0.055
-
 UDPgalactose
-
 Bos taurus
2.4.1.38 2.8
-
 agalactokeratan
-
 Bos taurus
2.4.1.38 21
-
 D-glucose
-
 Bos taurus
2.4.1.38 40
-
 N-acetylglucosamine
-
 Bos taurus
2.4.1.90 0.055
-
 UDPgalactose
-
 Bos taurus
2.4.1.90 2.8
-
 agalactokeratan
-
 Bos taurus
2.4.1.90 21
-
 D-glucose
-
 Bos taurus
2.4.1.90 40
-
 N-acetylglucosamine
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.38 soluble
-
 Bos taurus
-
-
2.4.1.90 soluble
-
 Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Co2+ activation at 14.9% of the activity with Mn2+ Bos taurus
2.4.1.38 Mn2+ required Bos taurus
2.4.1.38 Mn2+ maximal activity at 4 mM Bos taurus
2.4.1.38 Mn2+ Km for MnCl2: 0.34 mM Bos taurus
2.4.1.38 Zn2+ activation at 9.2% of the activity with Mn2+ Bos taurus
2.4.1.90 Co2+ activation at 14.9% of the activity with Mn2+ Bos taurus
2.4.1.90 Mn2+ required Bos taurus
2.4.1.90 Mn2+ maximal activity at 4 mM Bos taurus
2.4.1.90 Mn2+ Km for MnCl2: 0.34 mM Bos taurus
2.4.1.90 Zn2+ activation at 9.2% of the activity with Mn2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.38 42200
-
 x * 42200, SDS-PAGE Bos taurus
2.4.1.90 42200
-
 x * 42200, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.38 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides Bos taurus biosynthesis of keratan sulfate-like polysaccharides ?
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides Bos taurus biosynthesis of keratan sulfate-like polysaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Bos taurus
-
-
-
2.4.1.90 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38
-
 Bos taurus
2.4.1.90
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.38 cornea
-
 Bos taurus
-
2.4.1.38 milk
-
 Bos taurus
-
2.4.1.90 cornea
-
 Bos taurus
-
2.4.1.90 milk
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.38 10.7
-
-
 Bos taurus
2.4.1.90 10.7
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDP-galactose + methyl 2-acetamido-2-deoxy-beta-D-glucoside 76% of the activity with N-acetylglucosamine Bos taurus UDP + beta-D-galactosyl-1,4-beta-1-O-methyl-2-deoxy-2-acetylamido-beta-D-glucopyranoside
-
 ?
2.4.1.38 UDP-galactose + methyl 2-bromo-acetamido-2-deoxy-beta-D-glucoside 75% of the activity with N-acetylglucosamine Bos taurus UDP + beta-D-galactosyl-1,4-1-O-methyl-2-bromo-acetamido-2-deoxy-beta-D-glucoside
-
 ?
2.4.1.38 UDP-galactose + methyl 2-deoxy-2-benzamido-beta-D-glucoside 16% of the activity with N-acetylglucosamine Bos taurus UDP + beta-D-galactosyl-1,4-1-O-methyl-2-deoxy-2-benzamido-beta-D-glucoside
-
 ?
2.4.1.38 UDPgalactose + 2-acetamido-N-(L-aspart-4-oyl)-1,2-dideoxy-beta-glucoside 65% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + agalacto-ovomucoid 65% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + agalactokeratan agalactokeratan from bovine cornea and nasal septum, at 5% and 13% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + asialo-agalacto-alpha1-glycoprotein 42% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + di-acetylchitobiose 54% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + glucose in presence of alpha-lactalbumin Bos taurus lactose + UDP
-
 ?
2.4.1.38 UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide glycoproteins containing terminal nonreducing N-acetylglucosaminyl units Bos taurus UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosamine
-
 Bos taurus UDP + N-acetyllactosamine
-
 ?
2.4.1.38 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides biosynthesis of keratan sulfate-like polysaccharides Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside 67% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.38 UDPgalactose + tri-N-acetylchitotriose 64% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.90 UDP-galactose + methyl 2-acetamido-2-deoxy-beta-D-glucoside 76% of the activity with N-acetylglucosamine Bos taurus UDP + beta-D-galactosyl-1,4-beta-1-O-methyl-2-deoxy-2-acetylamido-beta-D-glucopyranoside
-
 ?
2.4.1.90 UDP-galactose + methyl 2-bromo-acetamido-2-deoxy-beta-D-glucoside 75% of the activity with N-acetylglucosamine Bos taurus UDP + beta-D-galactosyl-1,4-1-O-methyl-2-bromo-acetamido-2-deoxy-beta-D-glucoside
-
 ?
2.4.1.90 UDP-galactose + methyl 2-deoxy-2-benzamido-beta-D-glucoside 16% of the activity with N-acetylglucosamine Bos taurus UDP + beta-D-galactosyl-1,4-1-O-methyl-2-deoxy-2-benzamido-beta-D-glucoside
-
 ?
2.4.1.90 UDPgalactose + 2-acetamido-N-(L-aspart-4-oyl)-1,2-dideoxy-beta-glucoside 65% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + agalacto-ovomucoid 65% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + agalactokeratan agalactokeratan from bovine cornea and nasal septum, at 5% and 13% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + asialo-agalacto-alpha1-glycoprotein 42% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + di-acetylchitobiose 54% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + glucose in presence of alpha-lactalbumin Bos taurus lactose + UDP
-
 ?
2.4.1.90 UDPgalactose + N-acetyl-beta-D-glucosaminyl-glycopeptide glycoproteins containing terminal nonreducing N-acetylglucosaminyl units Bos taurus UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosamine
-
 Bos taurus UDP + N-acetyllactosamine
-
 ?
2.4.1.90 UDPgalactose + N-acetylglucosaminyl at the non-reducing ends of protein-bound oligosaccharides biosynthesis of keratan sulfate-like polysaccharides Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + p-nitrophenyl 2-acetamido-2-deoxy-beta-glucoside 67% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?
2.4.1.90 UDPgalactose + tri-N-acetylchitotriose 64% of the activity with N-acetylglucosamine Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.38 ? x * 42200, SDS-PAGE Bos taurus
2.4.1.90 ? x * 42200, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.38 7.5 10.5
-
 Bos taurus
2.4.1.90 7.5 10.5
-
 Bos taurus