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Literature summary extracted from

  • Henion, T.R.; Macher, B.A.; Anaraki, F.; Galili, U.
    Defining the minimal size of catalytically active primate alpha 1,3 galactosyltransferase: structure-function studies on the recombinant truncated enzyme (1994), Glycobiology, 4, 193-201.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.87 cloned from cDNA library, expression of full-length and truncated DNA in Escherichia coli and COS-1 cells, the truncated sequences are fused to protein A sequence resulting in expression of soluble chimeric proteins, DNA sequence determination and analysis Callithrix jacchus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.87 additional information truncation of 3 amino acid residues from the C-terminus, and a frame shift mutation result in a complete loss of activity Callithrix jacchus
2.4.1.87 N375V exchange and truncation of terminal V376, 70% loss of activity Callithrix jacchus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.87 additional information
-
 amino acid sequence alignment Callithrix jacchus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.87 Callithrix jacchus
-
 marmoset monkey
-
2.4.1.87 no activity in african green monkey
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.87 B95-8 cell
-
 Callithrix jacchus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.87 UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Callithrix jacchus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.87 UDP-galactose + N-acetyllactosamine i.e. beta-D-galactosyl-1,4-N-acetyl-D-glucosamine Callithrix jacchus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.4.1.87 alpha-1,3-galactosyltransferase
-
 Callithrix jacchus
2.4.1.87 alpha1,3GT
-
 Callithrix jacchus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.87 37
-
 assay at Callithrix jacchus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.87 8
-
 assay at Callithrix jacchus