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Literature summary extracted from

  • Van den Eijnden, D.H.; Winterwerp, H.; Smeeman, P.; Schiphorst, W.E.C.M.
    Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1-3 and beta 1-6 N-acetylglucosaminyltransferase activity (1983), J. Biol. Chem., 258, 3435-3437.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.149 CaCl2
-
 Rattus norvegicus
2.4.1.149 EDTA
-
 Rattus norvegicus
2.4.1.149 ZnCl2
-
 Rattus norvegicus
2.4.1.150 CaCl2
-
 Rattus norvegicus
2.4.1.150 EDTA
-
 Rattus norvegicus
2.4.1.150 ZnCl2
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.87 Mn2+ required Rattus norvegicus
2.4.1.149 Cd2+ CdCl2 slightly stimulates Rattus norvegicus
2.4.1.149 Mg2+ MgCl2 slightly stimulates Rattus norvegicus
2.4.1.149 Mn2+ MnCl2: 10-25 mM for optimal activity required Rattus norvegicus
2.4.1.150 Cd2+ CdCl2, slightly stimulates Rattus norvegicus
2.4.1.150 Mg2+ MgCl2, slightly stimulates Rattus norvegicus
2.4.1.150 Mn2+ MnCl2, 10-25 mM for optimal activity required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.87 additional information Rattus norvegicus functions in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells, attachs a galactose to the N-acetylglucosaminyl residue introduced by EC 2.4.1.149 ?
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R Rattus norvegicus might function in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group i antigenic structures, enzyme controls the synthesis of linear chain types by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R primer structure present on glycoprotein or glycolipid yielding a GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R sequence UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.150 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan Rattus norvegicus might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.87 Rattus norvegicus
-
 ascites fluid of Novikoff tumor cells maintained in rat
-
2.4.1.149 Rattus norvegicus
-
 Sprague Dawley rats
-
2.4.1.150 Rattus norvegicus
-
 Sprague Dawley rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.87 Novikoff ascites tumor cell
-
 Rattus norvegicus
-
2.4.1.149 Novikoff ascites tumor cell strain N1S1-67 Rattus norvegicus
-
2.4.1.150 Novikoff ascites tumor cell strain N1S1-67 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.87 additional information functions in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells, attachs a galactose to the N-acetylglucosaminyl residue introduced by EC 2.4.1.149 Rattus norvegicus ?
-
 ?
2.4.1.87 UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Rattus norvegicus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on asialo-alpha1-acid glycoproteins and other glycoproteins and oligosaccharides, GlcNAc residues are introduced to position C-3 of the terminal galactose of the glycoprotein Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R might function in the biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group i antigenic structures, enzyme controls the synthesis of linear chain types by adding a N-acetylglucosaminyl residue to a Galbeta(1-4)GlcNAc-R primer structure present on glycoprotein or glycolipid yielding a GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R sequence Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein GlcNAc residues are introduced to position C-3 of the terminal galactose of the glycoprotein, relative high activity towards asialo-alpha1-acid glycoprotein in Novikoff ascites tumor cells Rattus norvegicus UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + porcine submaxillary asialo-afuco-mucin poor acceptor, Galbeta(1-3)GalNAc as terminal acceptor structure Rattus norvegicus UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
 ?
2.4.1.150 UDP-N-acetyl-D-glucosamine + asialo-alpha1-acid glycoprotein acts on beta-galactosyl-1,4-N-acetylglucosaminyl-termini on asialo-alpha1-acid glycoproteins Rattus norvegicus UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
 ?
2.4.1.150 UDP-N-acetyl-D-glucosamine + asialo-alpha1-acid glycoprotein GlcNAc residues are introduced to position C-6 of the terminal galactose of the glycoprotein, relative high activity towards asialo-alpha1-acid glycoprotein in Novikoff ascites tumor cells Rattus norvegicus UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
 ?
2.4.1.150 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R acts on beta-galactosyl-1,4-N-acetylglucosaminyl-termini on asialo-alpha1-acid glycoproteins Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.150 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R GlcNAc residues are introduced to position C-6 of the terminal galactose of the glycoprotein Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.150 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosaminoglycan might function in biosynthesis of cell surface polylactosaminoglycans on Novikoff cells and blood group I antigenic structures, formation of the GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Gal-R branching points in the branched type of polylactosylaminoglycans Rattus norvegicus ?
-
 ?
2.4.1.150 UDP-N-acetyl-D-glucosamine + porcine submaxillary asialo-afuco-mucin poor acceptor, Galbeta(1-3)GalNAc as terminal acceptor structure Rattus norvegicus UDP + N-acetylglucosaminylated porcine submaxillary asialo-afuco-mucin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.149 37
-
 assay at Rattus norvegicus
2.4.1.150 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.149 6.5 8
-
 Rattus norvegicus
2.4.1.150 6.5 8
-
 Rattus norvegicus