BRENDA - Enzyme Database

Entamoeba histolytica: solubilization and biochemical characterization of dolichol phosphate mannose synthase, an essential enzyme in glycoprotein biosynthesis

Villagomez-Castro, J.C.; Calvo-Mendez, C.; Vargas-Rodriguez, L.; Flores-Carreon, A.; Lopez-Romero, E.; Exp. Parasitol. 88, 111-120 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
ADP
-
Entamoeba histolytica
2.4.1.83
AMP
-
Entamoeba histolytica
2.4.1.83
ATP
-
Entamoeba histolytica
2.4.1.83
GDP
strong
Entamoeba histolytica
2.4.1.83
GMP
-
Entamoeba histolytica
2.4.1.83
GTP
strong
Entamoeba histolytica
2.4.1.83
UDP
-
Entamoeba histolytica
2.4.1.83
UMP
-
Entamoeba histolytica
2.4.1.83
UTP
-
Entamoeba histolytica
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0041
-
GDPmannose
-
Entamoeba histolytica
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
divalent cation required for dolichol phosphate mannose: 2.5-7.5 mM Mg2+ or 2-3 mM Mn2+. Mg2+ is a better activator than Mn2+ in the reverse reaction
Entamoeba histolytica
2.4.1.83
Mn2+
divalent cation required for synthesis of dolichyl D-mannosyl phosphate: 2.5-7.5 mM Mg2+ or 2-3 mM Mn2+. Mg2+ is a better activator than Mn2+ in the reverse reaction
Entamoeba histolytica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
Entamoeba histolytica
essential enzyme in glycoprotein biosynthesis
GDP + dolichyl D-mannosyl phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Entamoeba histolytica
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489452
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
489452
Entamoeba histolytica
r
2.4.1.83
GDPmannose + dolichyl phosphate
essential enzyme in glycoprotein biosynthesis
489452
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.5
8
potassium phosphate or Tris-HCl buffer
Entamoeba histolytica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.83
6.5
8.5
pH 6.5: about 80% of maximal activity, pH 8.5: about 54% of maximal activity
Entamoeba histolytica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
ADP
-
Entamoeba histolytica
2.4.1.83
AMP
-
Entamoeba histolytica
2.4.1.83
ATP
-
Entamoeba histolytica
2.4.1.83
GDP
strong
Entamoeba histolytica
2.4.1.83
GMP
-
Entamoeba histolytica
2.4.1.83
GTP
strong
Entamoeba histolytica
2.4.1.83
UDP
-
Entamoeba histolytica
2.4.1.83
UMP
-
Entamoeba histolytica
2.4.1.83
UTP
-
Entamoeba histolytica
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0041
-
GDPmannose
-
Entamoeba histolytica
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mg2+
divalent cation required for dolichol phosphate mannose: 2.5-7.5 mM Mg2+ or 2-3 mM Mn2+. Mg2+ is a better activator than Mn2+ in the reverse reaction
Entamoeba histolytica
2.4.1.83
Mn2+
divalent cation required for synthesis of dolichyl D-mannosyl phosphate: 2.5-7.5 mM Mg2+ or 2-3 mM Mn2+. Mg2+ is a better activator than Mn2+ in the reverse reaction
Entamoeba histolytica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
Entamoeba histolytica
essential enzyme in glycoprotein biosynthesis
GDP + dolichyl D-mannosyl phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489452
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
489452
Entamoeba histolytica
r
2.4.1.83
GDPmannose + dolichyl phosphate
essential enzyme in glycoprotein biosynthesis
489452
Entamoeba histolytica
GDP + dolichyl D-mannosyl phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.83
7.5
8
potassium phosphate or Tris-HCl buffer
Entamoeba histolytica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.83
6.5
8.5
pH 6.5: about 80% of maximal activity, pH 8.5: about 54% of maximal activity
Entamoeba histolytica