BRENDA - Enzyme Database

The hydrophobic domain of dolichyl-phosphate-mannose synthase is not essential for enzyme activity or growth in Saccharomyces cerevisiae

Zimmerman, J.W.; Robbins, P.W.; J. Biol. Chem. 268, 16746-16753 (1993)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
additional information
mutant enzymes containing successive deletions or mutations of the hydrophobic region exhibit decreased transferase activity in vitro compared to the wild type enzyme, but the sequence is not essential for growth or for protein glycosylation. Although deletion of the entire hydrophobic region results in a soluble protein, mutant proteins containing 3 or 8 hydrophobic residues are still membrane-associated
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
endoplasmic reticulum
membrane
Saccharomyces cerevisiae
5783
-
2.4.1.83
membrane
of the endoplasmic reticulum
Saccharomyces cerevisiae
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489449
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489449
Saccharomyces cerevisiae
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.83
additional information
mutant enzymes containing successive deletions or mutations of the hydrophobic region exhibit decreased transferase activity in vitro compared to the wild type enzyme, but the sequence is not essential for growth or for protein glycosylation. Although deletion of the entire hydrophobic region results in a soluble protein, mutant proteins containing 3 or 8 hydrophobic residues are still membrane-associated
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
endoplasmic reticulum
membrane
Saccharomyces cerevisiae
5783
-
2.4.1.83
membrane
of the endoplasmic reticulum
Saccharomyces cerevisiae
16020
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489449
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489449
Saccharomyces cerevisiae
?