BRENDA - Enzyme Database

Activation of dolichyl-phospho-mannose synthase by phospholipids

Jensen, J.W.; Schutzbach, J.S.; Eur. J. Biochem. 153, 41-48 (1985)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
additional information
optimally active in a phospholipid matrix that contains some component phospholipids that prefer non-bilayer structural organization in isolation
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.4.1.83
unstable in presence of detergent
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
GDP
0.0013 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GMP
0.016 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GTP
0.003 mM, 50% inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0003
-
dolichyl phosphate
-
Rattus norvegicus
2.4.1.83
0.00069
-
GDPmannose
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mn2+
divalent cation required, maximal stimulation at 2.5 mM Mn2+
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.83
0.07
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489432
Rattus norvegicus
GDP + dolichyl D-mannosyl phosphate
-
489432
Rattus norvegicus
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.83
additional information
optimally active in a phospholipid matrix that contains some component phospholipids that prefer non-bilayer structural organization in isolation
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.1.83
unstable in presence of detergent
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.83
GDP
0.0013 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GMP
0.016 mM, 50% inhibition
Rattus norvegicus
2.4.1.83
GTP
0.003 mM, 50% inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.83
0.0003
-
dolichyl phosphate
-
Rattus norvegicus
2.4.1.83
0.00069
-
GDPmannose
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.83
Mn2+
divalent cation required, maximal stimulation at 2.5 mM Mn2+
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.83
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.83
0.07
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489432
Rattus norvegicus
GDP + dolichyl D-mannosyl phosphate
-
489432
Rattus norvegicus
?