BRENDA - Enzyme Database

Purification of GDP mannose:dolichyl-phosphate O-beta-D-mannosyltransferase from Saccharomyces cerevisiae

Haselbeck, A.; Eur. J. Biochem. 181, 663-668 (1989)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
endoplasmic reticulum
lumen
Saccharomyces cerevisiae
5783
-
2.4.1.83
membrane
enzyme is anchored with both its N-termini and C-termini in the membrane, while the main part of the protein is oriented towards the lumen of the endoplasmic reticulum
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.83
30000
-
x * 30000, SDS-PAGE
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.83
Saccharomyces cerevisiae
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.83
additional information
no glycosylation
Saccharomyces cerevisiae
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.83
0.475
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489428
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489428
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.83
?
x * 30000, SDS-PAGE
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.83
endoplasmic reticulum
lumen
Saccharomyces cerevisiae
5783
-
2.4.1.83
membrane
enzyme is anchored with both its N-termini and C-termini in the membrane, while the main part of the protein is oriented towards the lumen of the endoplasmic reticulum
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.83
30000
-
x * 30000, SDS-PAGE
Saccharomyces cerevisiae
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.83
additional information
no glycosylation
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.83
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.83
0.475
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.83
GDPmannose + dolichyl phosphate
-
489428
Saccharomyces cerevisiae
GDP + dolichyl D-mannosyl phosphate
-
489428
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.83
?
x * 30000, SDS-PAGE
Saccharomyces cerevisiae