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Literature summary extracted from

  • Marks, D.L.; Wu, K.; Paul, P.; Kamisaka, Y.; Watanabe, R.; Pagano, R.E.
    Oligomerization and topology of the Golgi membrane protein glucosylceramide synthase (1999), J. Biol. Chem., 274, 451-456.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.80 additional information deletion mutants, lacking the first 10 or the last 8 amino acid residues, show only 4% of the wild-type activity Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.1.80 labile enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.80 Trypsin
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.80 Golgi membrane
-
 Rattus norvegicus 139
-
2.4.1.80 microsome
-
 Homo sapiens
-
-
2.4.1.80 microsome associated with membranes, structural integrity of the microsomal membranes is essential for activity Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.80 additional information
-
 C- and N-terminal amino acid sequences Homo sapiens
2.4.1.80 additional information
-
 C- and N-terminal amino acid sequences Rattus norvegicus
2.4.1.80 additional information
-
 enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Homo sapiens
2.4.1.80 additional information
-
 enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Rattus norvegicus
2.4.1.80 38000
-
 x * 38000, SDS-PAGE Homo sapiens
2.4.1.80 38000
-
 x * 38000, native and recombinant enzyme, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine Homo sapiens
-
 UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine Rattus norvegicus initial key enzyme in glycosphingolipid biosynthesis UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.80 Homo sapiens
-
-
-
2.4.1.80 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.80 partial from Golgi membranes Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.80 brain
-
 Homo sapiens
-
2.4.1.80 brain
-
 Rattus norvegicus
-
2.4.1.80 keratinocyte
-
 Homo sapiens
-
2.4.1.80 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Homo sapiens UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Homo sapiens UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine initial key enzyme in glycosphingolipid biosynthesis Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay Rattus norvegicus UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.80 ? x * 38000, SDS-PAGE Homo sapiens
2.4.1.80 ? x * 38000, native and recombinant enzyme, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.80 GCS
-
 Homo sapiens
2.4.1.80 GCS
-
 Rattus norvegicus