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Literature summary extracted from

  • Martina, J.A.; Daniotti, J.L.; Maccioni, H.J.
    GM1 synthase depends on N-glycosylation for enzyme activity and trafficking to the Golgi complex (2000), Neurochem. Res., 25, 725-731.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.62 expression in CHO-K1 cells as fusion protein tagged with a nonapeptide from influenza virus hemagglutinin Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.62 additional information point mutation at the glycosylation site results in loss of activity and enzyme targeting to the endoplasmic reticulum instead of the Golgi apparatus Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.62 0.023
-
 N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine recombinant fusion protein Mus musculus
2.4.1.62 2.47
-
 UDP-galactose recombinant fusion protein Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.62 43000
-
 x * 43000, recombinant enzyme from CHO-K1 cells, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.62 UDP-galactose + ganglioside GA2 Mus musculus i.e. Cer-Glc-Gal-GalNAc UDP + ganglioside GA1 i.e. Cer-Glc-Gal-GalNAc-Gal ?
2.4.1.62 UDP-galactose + GD2 Mus musculus i.e. Cer-Glc-Gal-(GalNAc)(NeuNAc2) UDP + Gd1b i.e. Cer-Glc-Gal-(GalNAc-Gal)(NeuNAc2) ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine Mus musculus involved in biosynthesis of gangliosides UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine i.e. GM1 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.62 Mus musculus Q9Z0F0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.62 glycoprotein cDNA contains a variable number of glycosylation sites Mus musculus
2.4.1.62 glycoprotein Asn-linked high-mannose glycoprotein Mus musculus
2.4.1.62 glycoprotein inhibition of glycosylation by tunicamycin leads to an inactive enzyme and enzyme targeting to the endoplasmatic reticulum instead of the Golgi apparatus Mus musculus
2.4.1.62 additional information sensitive to cleavage by Endo H protease Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.62 0.000000051
-
 recombinant enzyme, crude cell homogenate of transfected CHO-K1 cells Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.62 UDP-galactose + ganglioside GA2 i.e. Cer-Glc-Gal-GalNAc Mus musculus UDP + ganglioside GA1 i.e. Cer-Glc-Gal-GalNAc-Gal ?
2.4.1.62 UDP-galactose + GD2 i.e. Cer-Glc-Gal-(GalNAc)(NeuNAc2) Mus musculus UDP + Gd1b i.e. Cer-Glc-Gal-(GalNAc-Gal)(NeuNAc2) ?
2.4.1.62 UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Mus musculus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine i.e. GM1 ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine involved in biosynthesis of gangliosides Mus musculus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine i.e. GM1 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.62 ? x * 43000, recombinant enzyme from CHO-K1 cells, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.62 GA1/GM1/GD1b synthase
-
 Mus musculus
2.4.1.62 Gal-T2
-
 Mus musculus