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Literature summary extracted from

  • Daniotti, J.L.; Martina, J.A.; Zurita, A.R.; Maccioni, H.J.
    Mouse beta 1,3-galactosyltransferase (GA1/GM1/GD1b synthase): protein characterization, tissue expression, and developmental regulation in neural retina (1999), J. Neurosci. Res., 58, 318-327.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.62 cloning and DNA sequence determination and analysis, expression in CHO-K1 and NIH-3T3 cells as fusion protein tagged with a nonapeptide from influenza virus hemagglutinin, amino acid sequence determination Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.62 0.023
-
 N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine recombinant fusion protein Mus musculus
2.4.1.62 2.47
-
 UDP-galactose recombinant fusion protein Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.62 Golgi apparatus recombinant enzyme, expressed in CHO-K1 cells Mus musculus 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.62 additional information
-
 amino acid sequence alignment Mus musculus
2.4.1.62 43000
-
 x * 43000, recombinant enzyme from CHO-K1 cells, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.62 UDP-galactose + ganglioside GA2 Mus musculus i.e. Cer-Glc-Gal-GalNAc UDP + ganglioside GA1 i.e. Cer-Glc-Gal-GalNAc-Gal ?
2.4.1.62 UDP-galactose + GD2 Mus musculus i.e. Cer-Glc-Gal-(GalNAc)(NeuNAc2) UDP + Gd1b i.e. Cer-Glc-Gal-(GalNAc-Gal)(NeuNAc2) ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine Mus musculus involved in biosynthesis of gangliosides UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine i.e. GM1 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.62 Mus musculus Q9Z0F0 single gene
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.62 glycoprotein N-glycosylated at the unique glycosylation site Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.62 brain
-
 Mus musculus
-
2.4.1.62 kidney
-
 Mus musculus
-
2.4.1.62 lung low activity content Mus musculus
-
2.4.1.62 neural retina expression level depends on the developmental stage Mus musculus
-
2.4.1.62 spleen
-
 Mus musculus
-
2.4.1.62 testis highest content of activity Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.62 0.00000005
-
 recombinant enzyme, crude cell homogenate of transfected NIH 3T3 cells Mus musculus
2.4.1.62 0.00000007
-
 recombinant enzyme, crude cell homogenate of transfected CHO-K1 cells Mus musculus
2.4.1.62 0.00000009
-
 testis, crude homogenate, native wild-type Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.62 UDP-galactose + ganglioside GA2 i.e. Cer-Glc-Gal-GalNAc Mus musculus UDP + ganglioside GA1 i.e. Cer-Glc-Gal-GalNAc-Gal ?
2.4.1.62 UDP-galactose + GD2 i.e. Cer-Glc-Gal-(GalNAc)(NeuNAc2) Mus musculus UDP + Gd1b i.e. Cer-Glc-Gal-(GalNAc-Gal)(NeuNAc2) ?
2.4.1.62 UDP-galactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine
-
 Mus musculus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine i.e. GM1 ?
2.4.1.62 UDPgalactose + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-1,4-beta-D-glucosyl-N-acylsphingosine involved in biosynthesis of gangliosides Mus musculus UDP + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosyl-N-acylsphingosine i.e. GM1 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.62 ? x * 43000, recombinant enzyme from CHO-K1 cells, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.62 GA1/GM1/GD1b synthase
-
 Mus musculus
2.4.1.62 Gal-T2
-
 Mus musculus