Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Myllylä, R.; Risteli, L.; Kivirikko, K.I.
    Assay of collagen-galactosyltransferase and collagen-glucosyltransferase activities and preliminary characterization of enzymic reactions with transferases from chick-embryo cartilage (1975), Eur. J. Biochem., 52, 401-410.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.66 additional information not activated by folate Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.50 additional information
-
 additional information kinetic study Gallus gallus
2.4.1.50 additional information
-
 additional information Km: 150 mg/ml, gelatinized calf skin collagen Gallus gallus
2.4.1.50 0.03
-
 UDPgalactose
-
 Gallus gallus
2.4.1.66 additional information
-
 additional information kinetic study Gallus gallus
2.4.1.66 additional information
-
 additional information Km: 14 mg/ml, gelatinized calf skin collagen Gallus gallus
2.4.1.66 0.03
-
 UDP-glucose
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.50 Co2+ 5.3% as effective as Mn2+ Gallus gallus
2.4.1.50 Fe2+ activates, 2.7% as effective as Mn2+ Gallus gallus
2.4.1.50 Mg2+ 12.2% as effective as Mn2+ Gallus gallus
2.4.1.50 Mn2+ requirement Gallus gallus
2.4.1.50 Mn2+ optimal concentration: 10 mM Gallus gallus
2.4.1.50 additional information not activated by Ca2+ Gallus gallus
2.4.1.50 additional information not activated by Cu2+ Gallus gallus
2.4.1.50 additional information not activated by Zn2+ Gallus gallus
2.4.1.50 additional information not activated by Ni2+ Gallus gallus
2.4.1.66 Ca2+ 26% as effective as Mn2+ Gallus gallus
2.4.1.66 Co2+ 32-33% as effective as Mn2+ Gallus gallus
2.4.1.66 Mg2+ activates, about 20% as effective as Mn2+ Gallus gallus
2.4.1.66 Mn2+ requirement Gallus gallus
2.4.1.66 Mn2+ 10 mM Gallus gallus
2.4.1.66 Mn2+ most effective metal cofactor Gallus gallus
2.4.1.66 additional information not activated by Ni2+ Gallus gallus
2.4.1.66 additional information not activated by Fe2+ Gallus gallus
2.4.1.66 additional information not activated by Zn2+, Cu2+ Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.50 Gallus gallus
-
-
-
2.4.1.66 Gallus gallus
-
 white Leghorn chicken
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.50 partial Gallus gallus
2.4.1.66 partial Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.50 cartilage embryo cartilage Gallus gallus
-
2.4.1.50 embryo embryo cartilage Gallus gallus
-
2.4.1.50 embryo 14-15 days old Gallus gallus
-
2.4.1.66 cartilage embryonic Gallus gallus
-
2.4.1.66 cartilage cartilage and cartilagenous bone Gallus gallus
-
2.4.1.66 embryo embryo cartilage Gallus gallus
-
2.4.1.66 embryo 14-15 days old Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.50 additional information no acceptor: free hydroxylysine Gallus gallus ?
-
 ?
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine dialyzable peptides prepared by collagenase digestion of bovine Achilles tendon collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine gelatinized insoluble calf skin collagen Gallus gallus UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen peptides prepared by collagenase-digestion from bovine Achilles tendon collagen Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?
2.4.1.66 UDP-glucose + 5-(D-galactosyloxy)-L-lysine-procollagen calf skin gelatin Gallus gallus UDP + 1,2-D-glucosyl-5-D-(galactosyloxy)-L-lysine-procollagen product is glucosylgalactosylhydroxylysine-collagen ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.50 37
-
 assay at Gallus gallus
2.4.1.66 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.50 7
-
 about Gallus gallus
2.4.1.66 7
-
-
 Gallus gallus