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Literature summary extracted from

  • Yokoyama, I.; Kobayashi, M.; Matsuda, K.
    Purification of the dimeric form of dextransucrase from Leuconostoc mesenteroides strains NRRL B-146 and B-1375 (1985), Agric. Biol. Chem., 49, 1385-1391.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.5 Dextrans addition of 0.5% exogenous dextran activates B-1416 enzyme 4.35fold, B-1375 enzyme is activated 2.76fold Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 130000 133000 disc gel electrophoresis Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 B-1375
-
2.4.1.5 Leuconostoc mesenteroides
-
 NRRL B-1416
-
2.4.1.5 Leuconostoc mesenteroides B-1375
-
 B-1375
-
2.4.1.5 Leuconostoc mesenteroides NRRL B-1416
-
 NRRL B-1416
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 B-1375 Leuconostoc mesenteroides
2.4.1.5 B-1416 Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 additional information
-
-
 Leuconostoc mesenteroides

Storage Stability

EC Number Storage Stability Organism
2.4.1.5 4°C, 0.1% bovine serum albumin, pH 5.2-8.5, stable for 24 h Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides NRRL B-1416 D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides B-1375 D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 dimer 2 * 64000-68000, SDS-PAGE after alkaline treatment, pH 10.5 Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 30
-
 B-1375 enzyme with dextran Leuconostoc mesenteroides
2.4.1.5 35
-
 B-1416 enzyme with and without dextran, B-1375 enzyme without dextran Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 35
-
 B-1375 enzyme stable Leuconostoc mesenteroides
2.4.1.5 40
-
 B-1416 enzyme is stable up to Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.2
-
-
 Leuconostoc mesenteroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.5 5.2 8.5 4°C, 0.1% bovine serum albumin, stable for 24 h, rapid inactivation below pH 5.0 Leuconostoc mesenteroides