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Literature summary extracted from

  • Kobayashi, M.; Matsuda, K.
    Purification and characterization of two activities of the intracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299 (1975), Biochim. Biophys. Acta, 397, 69-79.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.5 Dextrans addition of the B-1299 water-soluble dextan stimulates activity of enzyme I, no effect on activity of enzyme II Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 EDTA no effect Leuconostoc mesenteroides
2.4.1.5 Fe3+ enzyme I Leuconostoc mesenteroides
2.4.1.5 Guanidine-HCl 0.05 M, 60% inhibition Leuconostoc mesenteroides
2.4.1.5 Mn2+ enzyme I and II Leuconostoc mesenteroides
2.4.1.5 phenylmercuric acetate 1.0 mM, strong inhibition Leuconostoc mesenteroides
2.4.1.5 SDS 1.0 mM, strong inhibition Leuconostoc mesenteroides
2.4.1.5 Urea enzyme I loses 50% of its activity at 2.4 M urea, enzyme II is inhibited to 50% by 1.7 M urea Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.5 10.7
-
 sucrose enzyme I Leuconostoc mesenteroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.5 Co2+ 1 mM slight activation of enzyme I and II Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.5 69000
-
 1 * 69000, enzyme I, SDS-PAGE Leuconostoc mesenteroides
2.4.1.5 79000
-
 1 * 79000, enzyme II, SDS-PAGE Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 NRRL B-1299
-
2.4.1.5 Leuconostoc mesenteroides NRRL B-1299
-
 NRRL B-1299
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.5 2 forms: I and II Leuconostoc mesenteroides
2.4.1.5 NRRL B-1299 Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.5 additional information
-
-
 Leuconostoc mesenteroides
2.4.1.5 250
-
 enzyme II Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides NRRL B-1299 D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.5 monomer 1 * 69000, enzyme I, SDS-PAGE Leuconostoc mesenteroides
2.4.1.5 monomer 1 * 79000, enzyme II, SDS-PAGE Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.5 35
-
 enzyme I is stable for 10 min Leuconostoc mesenteroides
2.4.1.5 35 40 enzyme II Leuconostoc mesenteroides
2.4.1.5 45
-
 enzyme I Leuconostoc mesenteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.5 37 51 80% of activity maximum at 37°C and 51°C Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.5 35
-
 10 min, enzyme I stable Leuconostoc mesenteroides
2.4.1.5 45
-
 10 min, 80% loss of activity, enzyme I Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.5 5.5 6.9 enzyme II Leuconostoc mesenteroides
2.4.1.5 6.3 6.5 enzyme I Leuconostoc mesenteroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.5 4.6 7.2 4°C, 24 h, 50% loss of activity at pH 4.6 and 7.2, enzyme II Leuconostoc mesenteroides
2.4.1.5 4.7 8.5 4°C, 24 h, 50% loss of activity at pH 4.7 and 8.5, enzyme I Leuconostoc mesenteroides
2.4.1.5 5.3 5.8 4°C, 24 h, enzyme II stable Leuconostoc mesenteroides
2.4.1.5 6.2 6.9 4°C, 24 h, enzyme I stable Leuconostoc mesenteroides