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Literature summary extracted from

  • Kobayashi, M.; Yokoyama, I.; Matsuda, K.
    Substrate binding sites of Leuconostoc dextransucrase evaluated by inhibition kinetics (1986), Agric. Biol. Chem., 50, 2585-2590.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.5 6'-Amino-6'-deoxysucrose competitive Leuconostoc mesenteroides
2.4.1.5 D-gluconic acid
-
 Leuconostoc mesenteroides
2.4.1.5 D-glucono-1,5-lactone competitive Leuconostoc mesenteroides
2.4.1.5 D-glucuronic acid reduced, noncompetitive Leuconostoc mesenteroides
2.4.1.5 EDTA noncompetitive Leuconostoc mesenteroides
2.4.1.5 maltose
-
 Leuconostoc mesenteroides
2.4.1.5 methyl 6-amino-6-deoxyglucoside competitive Leuconostoc mesenteroides
2.4.1.5 methyl-alpha-D-glucoside competitive Leuconostoc mesenteroides
2.4.1.5 N-Methyl-D-glucamine competitive Leuconostoc mesenteroides
2.4.1.5 Nojirimycin noncompetitive Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.5 Leuconostoc mesenteroides
-
 NRRL B-1416
-
2.4.1.5 Leuconostoc mesenteroides NRRL B-1416
-
 NRRL B-1416
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?
2.4.1.5 sucrose + (1,6-alpha-D-glucosyl)n
-
 Leuconostoc mesenteroides NRRL B-1416 D-fructose + (1,6-alpha-D-glucosyl)n+1
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.5 0.2
-
 EDTA sucrose concentration 1.2-4.9 mM Leuconostoc mesenteroides
2.4.1.5 1.1
-
 EDTA sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 1.5
-
 Nojirimycin sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 6.3
-
 D-gluconic acid sucrose concentration 1.2-4.9 mM Leuconostoc mesenteroides
2.4.1.5 6.6
-
 6'-Amino-6'-deoxysucrose sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 7.5
-
 D-gluconic acid sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 9.4
-
 glucono-delta-lactone sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 9.5
-
 glucuronic acid sucrose concentration 1.2-4.9 mM Leuconostoc mesenteroides
2.4.1.5 12
-
 methyl-alpha-D-glucoside sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 13.9
-
 glucuronic acid sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 18
-
 6'-Amino-6'-deoxysucrose sucrose concentration 1.2-4.9 mM Leuconostoc mesenteroides
2.4.1.5 27.3
-
 Methyl-6-amino-6-deoxyglucoside sucrose concentration 1.2-4.9 mM Leuconostoc mesenteroides
2.4.1.5 30
-
 N-Methyl-D-glucamine sucrose concentration 12.2-97.4 mM Leuconostoc mesenteroides
2.4.1.5 68.3
-
 N-Methyl-D-glucamine sucrose concentration 1.2-4.9 mM Leuconostoc mesenteroides