Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marechal, E.; Miege, C.; Block, M.A.; Douce, R.; Joyard, J.
    The catalytic site of monogalactosyldiacylglycerol synthase from spinach chloroplast envelope membranes. Biochemical analysis of the structure and of the metal content (1995), J. Biol. Chem., 270, 5714-5722.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.46 dithiothreitol is essential to prevent loss of activity during purification Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.46 citraconic anhydride 0.01 and 0.05 mM, 47% and 97% inactivation after 30 min, respectively Spinacia oleracea
2.4.1.46 N-ethylmaleimide 5 mM, complete inactivation of enzyme in envelope membranes, 0.008 mM, complete inactivation of solubilized enzyme, preincubation with 1,2-dioleoylglycerol protects Spinacia oleracea
2.4.1.46 o-phenanthroline 0.1 mM, 55% inhibition, 1,2-dioleoylglycerol protects and activates enzyme Spinacia oleracea
2.4.1.46 tert-butyoxycarbonyl-L-methionine hydrosuccinimidyl ester 0.2 mM, almost complete inactivation after 30 min Spinacia oleracea
2.4.1.46 UDP
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.46 chloroplast envelope
-
 Spinacia oleracea 9941
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.46 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.46 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.46 0.026
-
 UDP UDPgalactose as the varied substrate Spinacia oleracea
2.4.1.46 0.052
-
 UDP dioleoylglycerol as the varied substrate Spinacia oleracea