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Literature summary extracted from

  • Coves, J.; Joyard, J.; Douce, R.
    Lipid requirement and kinetic studies of solubilized UDP-galactose:diacylglycerol galactosyltransferase activity from spinach chloroplast envelope membranes (1988), Proc. Natl. Acad. Sci. USA, 85, 4966-4970.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.46 Lipid addition of lipids extracted from chloroplast membranes is necessary to reveal activity of highly delipidated enzyme fraction Spinacia oleracea
2.4.1.46 phosphatidylglycerol best activator of partially purified, delipidated enzyme Spinacia oleracea
2.4.1.46 phosphatidylinositol activation of partially purified, delipidated enzyme Spinacia oleracea
2.4.1.46 sulfolipid activation of partially purified, delipidated enzyme Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.46 UDP competitive vs. UDP-galactose Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.46 0.1
-
 UDPgalactose
-
 Spinacia oleracea
2.4.1.46 1
-
 diacylglycerol
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.46 chloroplast envelope
-
 Spinacia oleracea 9941
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.46 UDPgalactose + 1,2-diacylglycerol Spinacia oleracea involved in biosynthesis of monogalactosyldiacylglycerol UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.46 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.46
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.46 UDP-galactose + 1,2-diacylglycerol
-
 Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?
2.4.1.46 UDPgalactose + 1,2-diacylglycerol involved in biosynthesis of monogalactosyldiacylglycerol Spinacia oleracea UDP + 3-beta-D-galactosyl-1,2-diacylglycerol
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.46 0.008
-
 UDP
-
 Spinacia oleracea