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Literature summary extracted from

  • Wragg, S.; Hagen, F.K.; Tabak, L.A.
    Identification of essential histidine residues in UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T1 (1997), Biochem. J., 328, 193-197.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 expression of wild-type and mutants in COS7 cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.41 H125A 1% of the wild-type activity Bos taurus
2.4.1.41 H211A no detectable activity Bos taurus
2.4.1.41 H341A 6% of the wild-type activity Bos taurus
2.4.1.41 H344A no detectable activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.41 diethyldicarbonate binds to His; hydroxylamine partiallly protects, UDP-GalNAc protects Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Bos taurus initial step of mucin-type O-glycosylation UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Bos taurus
-
 isozyme T1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.41 glycoprotein residues Asn-95 and Asn-552 of the recombinant enzyme are occupied by N-linked sugars in Cos7 cells Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide
-
 Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide initial step of mucin-type O-glycosylation Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?