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Literature summary extracted from

  • Wandall, H.H.; Hassan, H.; Mirgorodskaya, E.; Kristensen, A.K.; Roepstorff, P.; Bennett, E.P.; Nielsen, P.A.; Hollingsworth, M.A.; Burchell, J.; Taylor-Papadimitriou, J.; Clausen, H.
    Substrate specificities of three members of the human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase family, GalNAc-T1, -T2, and -T3 (1997), J. Biol. Chem., 272, 23503-23514.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 expression of GalNAc-T1, -T2 and -T3 as soluble proteins in insect Sf9 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 additional information
-
 additional information values for GalNAc-T1, -T2 and -T3 for different substrate peptides Homo sapiens
2.4.1.41 0.01
-
 UDP-GalNAc GalNAc-T2 Homo sapiens
2.4.1.41 0.027
-
 UDP-Gal GalNAc-T2 Homo sapiens
2.4.1.41 0.029
-
 UDP-GalNAc GalNAc-T3 Homo sapiens
2.4.1.41 0.062
-
 UDP-GalNAc GalNAc-T1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.41 soluble recombinant GalNAc-T1, -T2 and -T3, expressed in Sf9 cells Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Mn2+ requirement Homo sapiens
2.4.1.41 Mn2+ Mn2+ cannot be replaced by Mg2+ or Ca2+ Homo sapiens
2.4.1.41 additional information not activated by Mg2+ or Ca2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + fibronectin Homo sapiens fibronectin is a physiological substrate for GalNAc-T3 UDP + N-acetyl-D-galactosaminyl-fibronectin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Homo sapiens initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Homo sapiens
-
 GalNAc-T1, -T2 and -T3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 recombinant GalNAc-T1, -T2 and -T3, expressed in Sf9 cells Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.41 0.5
-
 GalNAc-T2 and -T3 Homo sapiens
2.4.1.41 0.6
-
 GalNAc-T1 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 additional information not: UDP-GlcNAc Homo sapiens ?
-
 ?
2.4.1.41 UDP-galactose + Muc2 peptide only GalNAc-T2, only with the Muc2 acceptor peptide Homo sapiens UDP + galactosyl-Muc2 peptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + fibronectin fibronectin is a physiological substrate for GalNAc-T3 Homo sapiens UDP + N-acetyl-D-galactosaminyl-fibronectin
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + HIV-V3 peptide HIVIIIBgp120, only GalNAc-T3 Homo sapiens UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide only GalNAc-T2 Homo sapiens UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide GalNAc-T1 and -T3: strict specificity for UDP-GalNAc, GalNAc-T2: utilizes UDP-GalNAc and UDP-Gal Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide 3 GalNAc-transferases: GalNAc-T1, -T2 and -T3 with distinct but partly overlapping specificities, dependent on the primary sequence of the acceptor substrate, acceptors: peptides based on Muc1 tandem repeat, TAP24, Muc2, Muc5AC, erythropoietin, hCG-beta, HIVIIIBgp120, ovine submaxillary mucin fragment, peptide derived from fibronectin, GalNAc-T1, -T2 and -T3 react at different rates with individual sites in the Muc1 repeat Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function Homo sapiens UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.41 30
-
 24 h, GalNAc-T1 and -T3: 40% loss of activity, GalNAc-T2: 60% loss of activity Homo sapiens