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Literature summary extracted from

  • Brockhausen, I.; Toki, D.; Brockhausen, J.; Peters, S.; Bielfeldt, T.; Kleen, A.; Paulsen, H.; Meldal, M.; Hagen, F.; Tabak, L.A.
    Specificity of O-glycosylation by bovine colostrum UDP-GalNAc: polypeptide alpha-N-acetylgalactosaminyltransferase using synthetic glycopeptide substrates (1996), Glycoconj. J., 13, 849-856.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 additional information
-
 additional information effect of substrate glycosylation and amino acid substitution of glycopeptides on Km-values and enzyme activity Bos taurus
2.4.1.41 1.3
-
 Ac-Pro-Thr-Thr-Thr-Pro-Ile-Ser-Thr-NH2 derived from the MUC2 repeat sequence Bos taurus
2.4.1.41 1.3
-
 Ac-Pro-Thr-Thr-Thr derived from the bovine myelin basic protein sequence Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Bos taurus catalyzes the first step in biosynthesis of O-linked oligosaccharides in many glycoproteins UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide Bos taurus catalyzes in vivo glycosylation of both threonine and serine UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Bos taurus
-
 colostrum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.41 colostrum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.41 30.6
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + Ac-Pro-Thr-Thr-Thr
-
 Bos taurus ?
-
 ?
2.4.1.41 UDP-N-acetyl-alpha-D-galactosamine + Ac-Pro-Thr-Thr-Thr-Pro-Ile-Ser-Thr-NH2
-
 Bos taurus ?
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide acceptors: synthetic glycopeptides and peptides most of which contain sequences derived from the tandem repeat region of MUC2 mucin Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide rate of incorporation of GalNAc into Thr is significantly greater than into Ser residues, presence of 1-2 GalNAc-Thr moieties in the subtrate reduces enzyme activity, especially when Galbeta1-3GalNAc is present, O-glycosylation depends on both amino acid sequence and prior glycosylation of substrates, enzyme is selective in glycosylating peptides with the Pro-Thr-Thr-Thr-Pro-Ile-Ser-Thr sequence in that the preferred primary site is the third Thr from the N-terminal end Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide catalyzes the first step in biosynthesis of O-linked oligosaccharides in many glycoproteins Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?
2.4.1.41 UDP-N-acetyl-D-galactosamine + polypeptide catalyzes in vivo glycosylation of both threonine and serine Bos taurus UDP + N-acetyl-D-galactosaminyl-polypeptide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.41 37
-
 assay at Bos taurus