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Literature summary extracted from

  • Schwyzer, M.; Hill, R.L.
    Porcine A blood group-specific N-acetylgalactosaminyltransferase. I. Purification from porcine submaxillary glands (1977), J. Biol. Chem., 252, 2338-2345.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.40 irreversible inactivation in absence of Triton X-100 Sus scrofa
2.4.1.40 loss of activity after freezing in buffer of pH 7.5 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.40 UMP
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.40 46000
-
 2 * 46000, sedimentation equilibrium of reduced and carboxymethylated enzyme Sus scrofa
2.4.1.40 100000
-
 zonal centrifugation, gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.40 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.40 glycoprotein
-
 Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.40
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.40 submaxillary gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.40 30
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.4.1.40 -70°C, 0.025 M MES buffer, pH 6.0, 1 mM EDTA, 0.03% Triton X-100, after removal of most of the buffer by dialysis, stable for 1 year Sus scrofa
2.4.1.40 4°C, pH 7.5, half-life is about 3 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.40 UDP-N-acetyl-D-galactosamine + asialo-porcine submaxillary mucin of A-negative blood-type
-
 Sus scrofa UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[asialo-porcine submaxillary mucin of A-negative blood-type]
-
 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
-
 Sus scrofa UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.40 dimer 2 * 46000, sedimentation equilibrium of reduced and carboxymethylated enzyme Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.40 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.40 6
-
 assay at Sus scrofa