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Literature summary extracted from

  • Takeya, A.; Hosomi, O.; Ishiura, M.
    Complete purification and characterization of alpha-3-N-acetylgalactosaminyltransferase encoded by the human blood group A gene (1990), J. Biochem., 107, 360-368.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.40 2'-fucosyllactose substrate inhibition above 1 mM Homo sapiens
2.4.1.40 lacto-N-fucopentaose I substrate inhibition above 2 mM Homo sapiens
2.4.1.40 UDP
-
 Homo sapiens
2.4.1.40 UDP-galactose
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.40 0.013
-
 UDP-GalNAc
-
 Homo sapiens
2.4.1.40 0.27
-
 2'-fucosyllactose
-
 Homo sapiens
2.4.1.40 0.35
-
 lacto-N-fucopentaose I
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.40 35000
-
 x * 35000, SDS-PAGE with and without 2-mercaptoethanol Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.40 Homo sapiens
-
 blood group A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.40 blood group A Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.40 blood plasma A Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.40 -20°C, rapid loss of activity Homo sapiens
2.4.1.40 -80°C, 20% loss of activity after 1 year Homo sapiens
2.4.1.40 4°C, 0.05 M Tris-HCl, pH 7.4, 2 mM MnCl2, 1 mM EDTA, 0.01% w/v Triton X-100, 0.2 M NaCl, 1 mM dithiothreitol, 0.03% w/v NaN3, 10% loss of activity per month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.40 additional information enzyme also catalyzes the transfer of galactose in alpha-linkage to 2'-fucosyllactose, the transfer rate of galactose is much lower than that of N-acetylgalactosamine Homo sapiens ?
-
 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose
-
 Homo sapiens UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose
-
 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose
-
 Homo sapiens UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose
-
 ?
2.4.1.40 UDP-N-acetyl-D-galactosamine + lacto-N-fucopentaose I i.e. Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc Homo sapiens UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[lacto-N-fucopentaose I]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.40 ? x * 35000, SDS-PAGE with and without 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.40 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.40 37
-
 30 min, 5% loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.40 6.6
-
-
 Homo sapiens