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Literature summary extracted from

  • Piro, G.; Zuppa, A.; Dalessandro, G.; Northcote, D.H.
    Glucomannan synthesis in pea epicotyls: the mannose and glucose transferases (1993), Planta, 190, 206-220.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.32 additional information not affected by spermine, spermidine, cellobiose, tunicamycin, bacitracin, EDTA and EGTA Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.32 ATP slight Pisum sativum
2.4.1.32 Ca2+ above 5 mM Pisum sativum
2.4.1.32 CHAPS 0.25-1% Pisum sativum
2.4.1.32 CHAPSO 0.25-1% Pisum sativum
2.4.1.32 Co2+ above 5 mM Pisum sativum
2.4.1.32 CTP slight Pisum sativum
2.4.1.32 cyclohexandione slight inactivation Pisum sativum
2.4.1.32 Digitonin slightly inhibitory at 1% Pisum sativum
2.4.1.32 formaldehyde 46% inactivation at 10 mM in presence of NaCNBH3 Pisum sativum
2.4.1.32 GDP
-
 Pisum sativum
2.4.1.32 GDP-D-glucose competitive Pisum sativum
2.4.1.32 GDP-L-fucose 40% inhibition at 1 mM Pisum sativum
2.4.1.32 glycerol slightly above 5% Pisum sativum
2.4.1.32 GMP
-
 Pisum sativum
2.4.1.32 GTP
-
 Pisum sativum
2.4.1.32 Mg2+ slightly inhibitory at 1-15 mM Pisum sativum
2.4.1.32 Mn2+ strong inhibition at 5 mM Pisum sativum
2.4.1.32 additional information not affected by N-acetylimidazol, acetyl-CoA, spermine, spermidine, cellobiose, tunicamycin, bacitracin, EDTA and EGTA Pisum sativum
2.4.1.32 N-ethylmaleimide concentration-dependent inactivation Pisum sativum
2.4.1.32 Phenylglyoxal concentration and time-dependent Pisum sativum
2.4.1.32 Phospholipase A2 fast inactivation, irreversible Pisum sativum
2.4.1.32 Phospholipase C slow inactivation, irreversible Pisum sativum
2.4.1.32 Triton X-100 0.25-1% Pisum sativum
2.4.1.32 UDP slight Pisum sativum
2.4.1.32 UTP slight Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.32 0.06
-
 GDP-D-mannose
-
 Pisum sativum
2.4.1.32 0.182
-
 GDP-D-mannose digitonin-solubilized enzyme Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.32 membrane
-
 Pisum sativum 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.32 Pisum sativum
-
 var. Alaska
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.32 seedling stem segment of third internode of etiolated seedling Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.32 additional information
-
 kinetics Pisum sativum
2.4.1.32 0.0017
-
 membrane-bound and digitonin-solubilized enzyme Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.32 GDP-D-mannose + (glucomannan)n glucomannan is soluble in 10% trichloroacetic acid and in water Pisum sativum GDP + (glucomannan)n+1 mannose attached by beta-1,4-linkages ?
2.4.1.32 GDP-D-mannose + (glucomannan)n glucomannan is soluble in 10% trichloroacetic acid and in water Pisum sativum GDP + (glucomannan)n+1 product analysis ?
2.4.1.32 GDP-D-mannose + (glucomannan)n alkali insoluble glucomannan Pisum sativum GDP + (glucomannan)n+1 mannose attached by beta-1,4-linkages ?
2.4.1.32 GDP-D-mannose + (glucomannan)n alkali insoluble glucomannan Pisum sativum GDP + (glucomannan)n+1 product analysis ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.32 30
-
-
 Pisum sativum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.32 additional information
-
 30% remaining activity at 2°C Pisum sativum
2.4.1.32 additional information
-
 cold-stress does not alter enzyme activity Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.32 0.001
-
 GDP-D-glucose digitonin-solubilized enzyme Pisum sativum
2.4.1.32 0.006
-
 GDP-D-glucose
-
 Pisum sativum