EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.27 | crystallization of the enzyme with UDPglucose and a 13mer DNA fragment containing an abasic site. Two crystal structures are obtained of a ternary complex BGT-UDP-DNA at 1.8 A and 2.5 A resolution, one with a Tris molecule and the other with a metal ion at the active site | Tequatrovirus T4 |
2.4.1.27 | ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site | Tequatrovirus T4 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.27 | UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA | Tequatrovirus T4 | the enzyme modifies T4-DNA | UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.27 | Tequatrovirus T4 | P04547 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.27 | UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA | base-flipping mechanism | Tequatrovirus T4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.27 | UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA | - |
Tequatrovirus T4 | UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? | |
2.4.1.27 | UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA | the enzyme modifies T4-DNA | Tequatrovirus T4 | UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? |